描述 | GM-CSF Protein, Human (CHO) is a hematopoietic growth factor and immune modulator. |
---|---|
研究背景 | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is produced by a variety of cell types including T cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. It is an important hematopoietic growth factor and immune modulator. GM-CSF also has profound effects on the functional activities of various circulating leukocytes. GM-CSF stimulates multipotent progenitor cells depending on its concentration, the proliferation of macrophage progenitors at the lowest doses, followed by granulocyte, erythroid, eosinophil, megakaryocyte and multipotent progenitors. It also stimulates the differentiation of myeloid leukemic cells and controls eosinophil function in some instances. GM-CSF also enhances the functionality of mature cells, such as neutrophils. In neutrophils, GM-CSF potentiates degranulation, the release of oxygen and nitrogen radical ions, phagocytosis, and inhibits apoptosis. GM-CSF inhibition in some animal models of autoimmune diseases showed significant beneficial effects. GM-CSF also has many pro-inflammatory functions and recent data implicates GM-CSF as a key factor in Th17 driven autoimmune inflammatory conditions. |
生物活性 | The ED50 is <0.2 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >5 × 106 units/mg. |
种属 | Human |
表达系统 | CHO |
标签 | Tag Free |
蛋白编号 | P04141 (A18-E144) |
基因 ID | |
同用名 | rHuGM-CSF; CSF-2; MGI-1GM; Pluripoietin-alpha; Molgramostin; Sargramostim |
氨基酸序列 | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE |
分子量 | 22-28 kDa |
纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
性状 | Lyophilized powder. |
组分 | Lyophilized after extensive dialysis against PBS. |
内毒素含量 | <0.2 EU/μg, determined by LAL method. |
复溶方法 | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). |
保存条件 & 期限 | Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
运输条件 | Room temperature in continental US; may vary elsewhere. |
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