IL-4 Protein, Human (HEK293, His) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells. IL-4 Protein, Human (HEK293, His) is a recombinant human interleukin-4 (rhIL-4) expressed in HEK 293 cells with a His tag.

Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM^[1].

1. The cell proliferation assay using TF-1 human erythroleukemic cells has an ED₅₀ value of 0.05-0.5 ng/mL.
2. Human IL-4-His can bind Human IL-4RA-Fc with an affinity constant of 0.99 nM.

Human

HEK293

C-6*His

P05112 (H25-S153)

3565  [NCBI]

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

18-21 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.