IGFBP2 Protein, Human (HEK293, His) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system.

Insulin-like Growth Factor Binding Protein 2 (IGFBP2) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system^[1]. Insulin-like Growth Factor-Binding Proteins (IGFBPs) modulate the actions of secreted insulin-like growth facts (IGFs) by binding to them and increase the IGF halflife in the extracellular milieu and circulation by sequestering them in this bound form. IGFBPs either enhance or inhibit IGF actions on target cells; the individual IGFBPs either inhibit or potentiate IGF effects on osteoblasts in bone. Human Insulin-like Growth Factor-Binding Protein 5 (rhIGFBP-5) is a unique and most abundant IGFBP stored in bone, having a high specific binding affinity for hydroxyapatite and extracellular matrix proteins, therefore fixing it and its bound IGFs within bone. rhIGFBP-5 consistently stimulates osteoblast cell proliferation in vitro, thus increasing the number of osteoblasts^[2].

1. The ED₅₀ is <2 µg/mL as measured by FDC-P1 cells.
2. Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1501 μg/ml in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 6.662×10³ units/mg.

• 
  Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1501 μg/ml in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 6.662×10³ units/mg.

Human

HEK293

C-His

P18065 (F40-Q325)

3485  [NCBI]

FRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQHHHHHH

Approximately 34.26 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.