描述 | IL-1 beta is a potent proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta plays a key role in inflammation and immunologic reactions. GMP IL-1 beta Protein, Human, is a recombinant GMP-grade protein, consists of 153 amino acids (A117-S269) and is expressed in E. coli. |
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研究背景 | Interleukin-1β (IL-1β) is one of the pro-inflammatory cytokines and is produced and secreted by a variety of cell types although the vast majority of studies have focussed on its production within cells of the innate immune system, such as monocytes and macrophages. |
体外研究 (In Vitro) | IL-1β (1-15625 pg/mL; 12 hours) dose-dependently increases production of the NF-κB-dependent inflammatory cytokine TNF-α, and a significant effect of IL-1β is observed at concentrations as low as 5 pg/mL in immortalized murine RAW264.7 macrophages. |
体内研究 (In Vivo) | IL-1β (8 ng; i.p.; daily; for 3 days) rescues Casp1−/− mice, restores survival and reduces lung bacterial load (i.e. days 0, 1, and 2 post-infection) in Chlamydia pneumoniae infected Casp1−/− mice. Yet, mice treated later (days 2, 3 and 4 post-infection) shows significantly increased bacterial counts relative to early-treated mice |
生物活性 | The specific activity is > 4×106 U/mg. |
种属 | Human |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P01584 (A117-S269) |
基因 ID | |
中文名 | 重组人细胞介素-1β,GMP级 |
同用名 | Interleukin-1 beta; Catabolin; IL1F2; IL1B |
氨基酸序列 | APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
分子量 | Approximately 17.0 kDa |
纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
性状 | Lyophilized powder |
组分 | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5. |
内毒素含量 | <0.05 EU/μg, determined by LAL method. |
复溶方法 | It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water. |
保存条件 & 期限 | Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
运输条件 | Room temperature in continental US; may vary elsewhere. |
文件资料 | |
参考文献 |
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