生物活性 | Measured by its binding ability in a functional ELISA. Immobilized VEGF-C-His at 2 μg/mL (100 μL/well) can bind VEGFR3-His and the EC50 is 1-10 ng/mL. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) and the ED50 is 0.1-0.5 μg/mL. |
---|---|
种属 | Human |
表达系统 | HEK293 |
标签 | C-His |
蛋白编号 | P49767 (T103-R227) |
基因 ID | |
同用名 | Flt4-L; vascular endothelial growth factor C; VEGFC; VRP |
分子量 | Approximately 22.5 kDa |
纯度 | Greater than 95% as determined by reducing SDS-PAGE |
性状 | Lyophilized powder. |
组分 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. |
内毒素含量 | <1 EU/μg, determined by LAL method. |
复溶方法 | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. |
保存条件 & 期限 | Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
运输条件 | Room temperature in continental US; may vary elsewhere. |
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