描述 | GH Protein, Human (CHO) is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration, which can be used for the treatment of growth disorders in children. |
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研究背景 | Recombinant Human Growth Hormone is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration, which can be used for the treatment of growth disorders in children. Growth Hormone is a stress hormone that stimulates production of IGF-1 and raises the concentration of glucose and free fatty acids. Growth Hormone exerts some of its effects by binding to receptors on target cells, where it activates the MAPK/ERK pathway |
生物活性 | The ED50 is <0.5 ng/mL as measured by murine Balb/c 3T3 cells, corresponding to a specific activity of >2 × 106 units/mg. |
种属 | Human |
表达系统 | CHO |
标签 | Tag Free |
蛋白编号 | P01241 (F27-F217) |
基因 ID | |
中文名 | 重组人生长激素 (CHO 细胞表达) |
同用名 | rHuGH; Somatotropin; GH-N; GH1 |
氨基酸序列 | FPTIPLSRLFDNASLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF |
分子量 | 20-24 kDa |
纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
性状 | Lyophilized powder. |
组分 | Lyophilized after extensive dialysis against PBS. |
内毒素含量 | <0.2 EU/μg, determined by LAL method. |
复溶方法 | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). |
保存条件 & 期限 | Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
运输条件 | Room temperature in continental US; may vary elsewhere. |
文件资料 | |
参考文献 |
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