3x DYKDDDDK Tag TFA|西域试剂

3x DYKDDDDK Tag TFA,98.10%

产品编号：Bellancom-P3332A| 分子式：C₁₂₅H₁₇₇F₃N₃₀O₆₀| 分子量：3116.90

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-P3332A	￥675.00	杭州北京（现货）
Bellancom-P3332A	￥1980.00	杭州北京（现货）
Bellancom-P3332A	￥3240.00	杭州北京（现货）

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3x DYKDDDDK Tag TFA

产品介绍

DYKDDDDK peptide (FLAG) 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常，它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。

生物活性

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance .

体外研究

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

H₂O : ≥ 100 mg/mL (32.08 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.3208 mL	1.6042 mL	3.2083 mL
5 mM	0.0642 mL	0.3208 mL	0.6417 mL
10 mM	0.0321 mL	0.1604 mL	0.3208 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

参考文献