Janelia Fluor? 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor? 646, Azide can be used for live-cell imaging experiments. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Storage: protect from light.

Maximum absorption wavelength (λabs)= 646 nm, maximum emission wavelength (λem)=664 nm.
JF646 can serve as a ligand for self-labeling tag (such as HaloTag).
Janelia Fluor® 646, Azide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403.  [Content Brief]

  . Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28.  [Content Brief]