Apamin TFA Apamine TFA|西域试剂

Apamin TFA Apamine TFA,96.44%

产品编号：Bellancom-P0256A| 分子式：C₈₁H₁₃₂F₃N₃₁O₂₆S₄| 分子量：2141.36

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-P0256A		￥1500.00	杭州北京（现货）
Bellancom-P0256A		￥2500.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

Apamin TFA Apamine TFA

产品介绍

Apamin TFA (Apamine TFA) 是在蜂毒素中发现的一种 18 个氨基酸的肽类神经毒素，是一种 Ca²⁺ 激活的 K⁺ (SK) 通道的特异性和选择性的阻断剂，具有抗炎和抗纤维化的作用。

生物活性

Apamin TFA (Apamine TFA) is an 18 amino acid peptide neurotoxin found in apitoxin (bee venom), is known as a specifically selective blocker of Ca²⁺-activated K⁺ (SK) channels and exhibits anti-inflammatory and anti-fibrotic activity.

体外研究

Apamin (0.5-2 µg/mL; 24 hours; HSC-T6 cells) treatment markedly reduces the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Apamin treatment abrogats the activation of p-Smad2/3 and Smad4 induced by TGF-β1.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	HSC-T6 cells
Concentration:	0.5 µg/mL, 1 µg/mL and 2 µg/mL
Incubation Time:	24 hours
Result:	Markedly reduced the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Abrogated the activation of p-Smad2/3 and Smad4 induced by TGF-β1.

体内研究
(In Vivo)

Apamin (0.1 mg/kg; intraperitoneal injection; twice a week; for 4 weeks; C57BL/6 male mice) treatment results in decreased liver injury and proinflammatory cytokine levels. Apamin suppresses the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-fed mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-week-old C57BL/6 male mice (20-25 g) with DDC feeding
Dosage:	0.1 mg/kg
Administration:	Intraperitoneal injection; twice a week; for 4 weeks
Result:	Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-week-old C57BL/6 male mice (20-25 g) with DDC feeding
Dosage:	0.1 mg/kg
Administration:	Intraperitoneal injection; twice a week; for 4 weeks
Result:	Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-week-old C57BL/6 male mice (20-25 g) with DDC feeding
Dosage:	0.1 mg/kg
Administration:	Intraperitoneal injection; twice a week; for 4 weeks
Result:	Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.

性状

Solid

溶解性数据

In Vitro:

H₂O : 50 mg/mL (23.35 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.4670 mL	2.3350 mL	4.6699 mL
5 mM	0.0934 mL	0.4670 mL	0.9340 mL
10 mM	0.0467 mL	0.2335 mL	0.4670 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶