β-Amyloid (1-42), (rat/mouse) (TFA) Amyloid β-peptide (1-42 (rat/mouse) TFA)|西域试剂

β-Amyloid (1-42), (rat/mouse) (TFA) Amyloid β-peptide (1-42 (rat/mouse) TFA),95.84%

产品编号：Bellancom-P1388A| 分子式：C₁₉₉H₃₀₇N₅₃O₅₉S.C₂HF₃O₂| 分子量：4532.04

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货号	价格	库存与货期
Bellancom-P1388A	￥2000.00	杭州北京（现货）
Bellancom-P1388A	￥3200.00	杭州北京（现货）
Bellancom-P1388A	￥12000.00	杭州北京（现货）

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β-Amyloid (1-42), (rat/mouse) (TFA) Amyloid β-peptide (1-42 (rat/mouse) TFA)

产品介绍

β-Amyloid (1-42), (rat/mouse) TFA是由 42 个氨基酸组成的多肽，对海马切片有毒性，可用于阿尔兹海默症的研究。

生物活性

β-Amyloid (1-42), (rat/mouse) TFA is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.

体外研究

β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.
2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.
3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
4. Next, the solution was aged 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.
Methods vary depends on the downstream applications.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 55 mg/mL (12.14 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.2207 mL	1.1033 mL	2.2065 mL
5 mM	0.0441 mL	0.2207 mL	0.4413 mL
10 mM	0.0221 mL	0.1103 mL	0.2207 mL

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (0.55 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (0.55 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。