TAT-DEF-Elk-1 TFA TDE TFA|西域试剂

TAT-DEF-Elk-1 TFA TDE TFA

产品编号：Bellancom-P2262A| 分子式：C₁₅₇H₂₆₀N₅₇F₃O₄₂| 分子量：3675.09

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货号	价格	库存与货期
Bellancom-P2262A	￥1500.00	杭州北京（现货）
Bellancom-P2262A	￥4500.00	杭州北京（现货）
Bellancom-P2262A	￥7500.00	杭州北京（现货）

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TAT-DEF-Elk-1 TFA TDE TFA

产品介绍

TAT-DEF-Elk-1 TFA (TDE TFA) 是 Elk-1 的一种可穿透细胞的肽抑制剂，通过模仿和特异性干扰 Elk-1 的 DEF 结构域来实现抑制。TAT-DEF-Elk-1 TFA阻止 Elk-1 磷酸化并防止 Elk-1 核易位，且不会干扰 ERK 或 MSK1 活化。TAT-DEF-Elk-1 TFA 是分析神经元可塑性发展过程中 Elk-1 在此过程中的作用的有用工具。

生物活性

TAT-DEF-Elk-1 TFA (TDE TFA) is a cell-penetrating peptide inhibitor of Elk-1, mimics and specifically interferes with the DEF domain of Elk-1. TAT-DEF-Elk-1 TFA blocks Elk-1 phosphorylation and prevents Elk-1 nuclear translocation without interfering with ERK nor MSK1 activation. TAT-DEF-Elk-1 TFA is a useful tool to analyze the role of Elk-1 in this process during the development of neuronal plasticity.

体外研究

Elk-1 phosphorylation on Ser383/389 has a dual function and triggers both Elk-1 nuclear translocation and SRE-dependent gene expression.
TAT-DEF-Elk-1 TFA (5 μM; 1 hour) specifically inhibits glutamate-induced elk-1 activation and does not interfer with ERK, MSK-1, or CREB phosphorylation.
TAT-DEF-Elk-1 TFA (5-10 μM; 2 hour) treatment shows a significant inhibition of c-Fos, Zif268 and JunB, but has no effects on c-Jun expression.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	Neurons
Concentration:	5 μM; 10 μM
Incubation Time:	1 hour
Result:	Decreased elk-1 expression and had no effects on ERK, MSK-1, or CREB phosphorylation.

RT-PCR

Cell Line:	Primary striatal neurons
Concentration:	5 μM
Incubation Time:	2 hour
Result:	Decreased c-Fos, Zif268 and JunB mRNA level but did not effect c-Jun.

体内研究
(In Vivo)

TAT-DEF-Elk-1 TFA (intraperitoneal injection; 1mg/kg; daily; 14 days) reflects antidepressant efficacy in mice, it decreases immobility similar to the reference antidepressants fluoxetine and desipramine (DMI).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl6 mice (3-6 months old males) are subjected to social defeat stress
Dosage:	1 mg/kg
Administration:	Intraperitoneal injection; daily; 14 days
Result:	Reversed social-defeat induced decrease of hippocampal Bdnf expression by repeated TDE administration.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl6 mice (3-6 months old males) are subjected to social defeat stress
Dosage:	1 mg/kg
Administration:	Intraperitoneal injection; daily; 14 days
Result:	Reversed social-defeat induced decrease of hippocampal Bdnf expression by repeated TDE administration.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl6 mice (3-6 months old males) are subjected to social defeat stress
Dosage:	1 mg/kg
Administration:	Intraperitoneal injection; daily; 14 days
Result:	Reversed social-defeat induced decrease of hippocampal Bdnf expression by repeated TDE administration.