Pep2m, myristoylated TFA Myr-Pep2m TFA|西域试剂

Pep2m, myristoylated TFA Myr-Pep2m TFA,99.77%

产品编号：Bellancom-P1399A| 分子式：C₆₅H₁₁₉F₃N₁₈O₁₆S| 分子量：1497.83

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-P1399A		￥3500.00	杭州北京（现货）

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Pep2m, myristoylated TFA Myr-Pep2m TFA

产品介绍

Pep2m, myristoylated TFA (Myr-Pep2m TFA) 是一种细胞通透性肽。Pep2m, myristoylated TFA 能破坏蛋白激酶 ζ (PKMζ) 下游靶点 NSF/GluR2 相互作用。PKMζ 具有自主活性的 PKC 同工酶。

生物活性

Pep2m, myristoylated TFA (Myr-Pep2m TFA) is a cell-permeable peptide. Pep2m, myristoylated TFA can disrupt the protein kinase ζ (PKMζ) downstream targets, N-ethylmaleimide-sensitive factor/glutamate receptor subunit 2 (NSF/GluR2) interactions. PKMζ is an autonomously active isozyme of protein kinase C (PKC).

体外研究

Pep2m, myristoylated TFA (10 μM) blocks PKMζ-mediated AMPA receptor (AMPAR) potentiation.
Pep2m, myristoylated TFA does not block the increase of PKMζ in the hippocampal slices during long-term potentiation (LTP) maintenance, indicating that blocking NSF/GluR2 interactions do not prevent the induction of PKMζ synthesis.
Pep2m, myristoylated TFA blocks NSF/GluR2-mediated AMPAR trafficking, and reverses persistent potentiation at both the strongly stimulates synapses and the weakly stimulats synapses that underwent synaptic tagging and capture.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

Pep2m, myristoylated TFA (10 µg/20 µL) results in an increase in paw withdrawal thresholds (PWTs) on nociceptive responses in the formalin test.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female and male Long-Evans hooded rats (8 weeks)
Dosage:	10 µg (in 20 µL)
Administration:	Intrathecal injection
Result:	Resulted in an increase in PWTs, in both male and female rats at various time points tested.

体内研究

Pep2m, myristoylated TFA (10 µg/20 µL) results in an increase in paw withdrawal thresholds (PWTs) on nociceptive responses in the formalin test.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female and male Long-Evans hooded rats (8 weeks)
Dosage:	10 µg (in 20 µL)
Administration:	Intrathecal injection
Result:	Resulted in an increase in PWTs, in both male and female rats at various time points tested.

性状

Solid

溶解性数据

In Vitro:

DMSO : 33.33 mg/mL (22.25 mM; Need ultrasonic)

H₂O : 3.33 mg/mL (2.22 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.6676 mL	3.3382 mL	6.6763 mL
5 mM	0.1335 mL	0.6676 mL	1.3353 mL
10 mM	0.0668 mL	0.3338 mL	0.6676 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (1.67 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (1.67 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (1.67 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (1.67 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。