Psalmotoxin 1 PcTx1; Psalmopoeus cambridgei toxin-1|西域试剂

Psalmotoxin 1 PcTx1; Psalmopoeus cambridgei toxin-1,99.0%

产品编号：Bellancom-P1411| 分子式：C₂₀₀H₃₁₂N₆₂O₅₇S₆| 分子量：4689.41

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-P1411		￥7000.00	杭州北京（现货）

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Psalmotoxin 1 PcTx1; Psalmopoeus cambridgei toxin-1

产品介绍

Psalmotoxin 1 (PcTx1) 是一种蛋白质毒素，可与酸敏感离子通道 1a (ASIC1a) 的亚基-亚基界面结合。Psalmotoxin 1 通过增加 ASIC1a 对 H⁺ 的表观亲和力 (apparent affinity) 来有效地、选择性抑制 ASIC1a (IC₅₀: 0.9 nM)。Psalmotoxin 1 可诱导细胞凋亡 (apoptosis)，抑制癌细胞迁移、增殖和侵袭。Psalmotoxin 1 可用于癌症或神经系统疾病的研究。

生物活性

Psalmotoxin 1 (PcTx1) is a protein toxin that can bind at subunit-subunit interfaces of acid-sensing ion channel 1a (ASIC1a). Psalmotoxin 1 is a potent and slective ASIC1a inhibitor (IC₅₀: 0.9 nM) by increasing the apparent affinity for H⁺ of ASIC1a. Psalmotoxin 1 can induce cell apoptosis, also inhibits cell migration, proferliration and invasion of cancer cells. Psalmotoxin 1 can be used in the research of cancers, or neurological disease^[4]^[6].

体外研究

Psalmotoxin 1 (20 nM, 125 s) inhibits ASIC1a currents by drastically shifting the steady-state desensitization curve to lower H⁺ concentrations.
Psalmotoxin 1 (30 nM) competes with Ca²⁺ in binding to ASIC1a channels.
Psalmotoxin 1 (100 or 200 ng, 24-72 h) significantly weakens the migration, proliferation and invasion of MCF-7 and MDA-MB-231 cells^[4].
Psalmotoxin 1 (100 ng/mL, 24 h) significantly inhibits acid-induced increases in intracellular calcium and LDH release, induces cell apoptosis and cell cycle arrest in nucleus pulposus cells (NPCs)^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[4]

Cell Line:	MCF-7 and MDA-MB-231 cells
Concentration:	100 or 200 ng
Incubation Time:	24, 48, 72 h
Result:	Inhibited the cell migration, proliferation and invasion of breast cancer cells.

Western Blot Analysis^[5]

Cell Line:	Nucleus pulposus cells (NPCs)
Concentration:	100 ng/mL
Incubation Time:	24 h
Result:	Decreased Bax and cleaved caspase-3 expression, and increased Bcl-2 expression.

体内研究
(In Vivo)

Psalmotoxin 1 (i.c.v., 1 ng/kg, a single dose) is neuroprotective in a conscious model of stroke via direct inhibition of ASIC1a.
Psalmotoxin 1 (tail vein injection, 10 ng/kg, daily for 7 days) inhibits tumor growth in breast cancer mice model^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male spontaneously hypertensive rats (SHR)
Dosage:	1 ng/kg, a single dose.
Administration:	Intracerebroventricular (i.c.v.) injection
Result:	Reduced cortical and striatal infarct volumes measured 72 h post-stroke. Reduced the severity of motor deficit at 1 and 3 days after stroke compared to control rats. Displayed an anti-apoptotic effect in the occluded hemisphere (reduced stroke-induced caspase-3 positive cells).

Animal Model:	Female nude BALB/C mice (orthotopic implantation, MCF-7 and MDA-MB-231 cells)
Dosage:	10 ng/kg, daily for 7 days.
Administration:	Tail vein injection
Result:	Inhibited breast tumor growth.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male spontaneously hypertensive rats (SHR)
Dosage:	1 ng/kg, a single dose.
Administration:	Intracerebroventricular (i.c.v.) injection
Result:	Reduced cortical and striatal infarct volumes measured 72 h post-stroke. Reduced the severity of motor deficit at 1 and 3 days after stroke compared to control rats. Displayed an anti-apoptotic effect in the occluded hemisphere (reduced stroke-induced caspase-3 positive cells).

Animal Model:	Female nude BALB/C mice (orthotopic implantation, MCF-7 and MDA-MB-231 cells)
Dosage:	10 ng/kg, daily for 7 days.
Administration:	Tail vein injection
Result:	Inhibited breast tumor growth.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male spontaneously hypertensive rats (SHR)
Dosage:	1 ng/kg, a single dose.
Administration:	Intracerebroventricular (i.c.v.) injection
Result:	Reduced cortical and striatal infarct volumes measured 72 h post-stroke. Reduced the severity of motor deficit at 1 and 3 days after stroke compared to control rats. Displayed an anti-apoptotic effect in the occluded hemisphere (reduced stroke-induced caspase-3 positive cells).

Animal Model:	Female nude BALB/C mice (orthotopic implantation, MCF-7 and MDA-MB-231 cells)
Dosage:	10 ng/kg, daily for 7 days.
Administration:	Tail vein injection
Result:	Inhibited breast tumor growth.