IRBP (1-20), human TFA,98.55%

产品编号:Bellancom-P1587A| 分子式:C103H165F3N24O30S| 分子量:2308.62

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Bellancom-P1587A
1800.00 杭州 北京(现货)
Bellancom-P1587A
4800.00 杭州 北京(现货)
Bellancom-P1587A
8000.00 杭州 北京(现货)

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IRBP (1-20), human TFA

产品介绍 IRBP (1-20), human TFA 包含 H-2b 单倍型的主要表位。IRBP (1-20), human TFA 诱导H-2b 小鼠实验性自身免疫性葡萄膜视网膜炎 (EAU)。
生物活性

IRBP (1-20), human TFA contains a major epitope for the H-2b haplotype. IRBP (1-20), human TFA induces experimental autoimmune uveoretinitis (EAU) in H-2b mice.

体外研究
体内研究

IRBP (1-20), human TFA induces EAU.
For determination of proliferative and cytokine responses, lymph node and spleen cells are collected 21 days after immunization and are stimulated in culture with the appropriate antigen. Cells of C57BL/6 mice and 129/J mice immunized with IRBP (1-20), human TFA proliferated in response to the peptide.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (H-2b) mice, H-2b (C57BL/6, 129/J) mice
Dosage: 200, 300 μg
Administration: I.h.; After 21 days
Result: C57BL/6 (H-2b) mice immunized with the human peptide (200-300 μg= 80-120 nmoles) developed EAU. 129/J mice (H-2b) also developed disease, albeit with a lower average score than C57BL/6.
体内研究

IRBP (1-20), human TFA induces EAU.
For determination of proliferative and cytokine responses, lymph node and spleen cells are collected 21 days after immunization and are stimulated in culture with the appropriate antigen. Cells of C57BL/6 mice and 129/J mice immunized with IRBP (1-20), human TFA proliferated in response to the peptide.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (H-2b) mice, H-2b (C57BL/6, 129/J) mice
Dosage: 200, 300 μg
Administration: I.h.; After 21 days
Result: C57BL/6 (H-2b) mice immunized with the human peptide (200-300 μg= 80-120 nmoles) developed EAU. 129/J mice (H-2b) also developed disease, albeit with a lower average score than C57BL/6.
性状Solid
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (43.32 mM; Need ultrasonic)

H2O : 8.33 mg/mL (3.61 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.4332 mL 2.1658 mL 4.3316 mL
5 mM 0.0866 mL 0.4332 mL 0.8663 mL
10 mM 0.0433 mL 0.2166 mL 0.4332 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: 2.5 mg/mL (1.08 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (1.08 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 西域 网站选购。
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

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