LAH4 TFA|西域试剂

LAH4 TFA,99.17%

产品编号：Bellancom-P0311A| 分子式：C₁₃₄H₂₂₉F₃N₃₈O₂₉| 分子量：2893.55

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-P0311A	￥2500.00	杭州北京（现货）
Bellancom-P0311A	￥5800.00	杭州北京（现货）
Bellancom-P0311A	￥9600.00	杭州北京（现货）

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LAH4 TFA

产品介绍

LAH4 TFA 是两亲性肽抗生素的 α 螺旋，具有强大的抗菌 (antimicrobial)、核酸转染(nucleic acid transfection) 和细胞渗透活性。LAH4 TFA 具有较高的质粒 DNA 传递能力。LAH4 TFA 对细菌膜外膜中的阴离子脂质有很强的亲和力。

生物活性

LAH4 TFA, an alpha-helix of the designed amphipathic peptide antibiotic, exhibits potent antimicrobial, nucleic acid transfection and cell penetration activities. LAH4 TFA possesses high plasmid DNA delivery capacities. LAH4 TFA has a strong affinity for anionic lipids found in the outer membrane of bacterial membranes.

体外研究

LAH4 TFA is capable of complexing DNA, associating with the cell surfacemembrane and then, when enveloped within an endosome, disrupts the endosomal membrane as the pH drops.
LAH4 TFA possesses robust plasmid DNA transfection properties. Peptides of the LAH4 family are able to efficiently deliver siRNAs in vitro into a human cell line.
LAH4 TFA is found to mediate the intracellular delivery of both protein and nucleotide cargo and facilitate protein internalization using mechanisms involving endosomal acidification and processing through the proteasome pathway, leading to enhanced cross presentation of protein antigen by dendritic cells to CD8+ T cells. LAH4 TFA also improves the internalization of CpG, resulting in NF-kB activation, thus potentiating the adjuvant effect of CpG.
LAH4 TFA exhibits antibiotic activities against Escherichia coli and Bacillus subtilis. the peptide does not, however, lyse human red blood cells at bacteriocidal concentrations. The antibiotic activities of LAH4 TFA are 2 orders of magnitude more pronounced at pH 5 when compared with pH 7.5^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

H₂O : 100 mg/mL (34.56 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.3456 mL	1.7280 mL	3.4560 mL
5 mM	0.0691 mL	0.3456 mL	0.6912 mL
10 mM	0.0346 mL	0.1728 mL	0.3456 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶