ccc_R08|西域试剂

ccc_R08,98.87%

产品编号：Bellancom-148560| 分子式：C₂₂H₁₉ClO₆| 分子量：414.84

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-148560	￥3000.00	杭州北京（现货）
Bellancom-148560	￥4500.00	杭州北京（现货）
Bellancom-148560	￥9500.00	杭州北京（现货）
Bellancom-148560	￥15000.00	杭州北京（现货）

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ccc_R08

产品介绍

ccc_R08 是一种无细胞毒性且口服有效的 cccDNA 抑制剂，可降低 HBV 感染小鼠肝脏中的 cccDNA 水平。ccc_R08 可用于 HBV病毒 (乙型肝炎病毒) 感染的研究。

生物活性

ccc_R08 is a non-cytotoxic and orally active cccDNA inhibitor that reduces cccDNA levels in the liver of HBV-infected mice. ccc_R08 can be used in the study of HBV virus (hepatitis B virus) infection.

体外研究

ccc_R08 (0.3, 1.0, 3.2, 10, 32 µM; 5 days) significantly reduces the level of cccDNA, protein-free RC-DNA, and double stranded linear DNA (DL-DNA) in HepDES19 cells.
ccc_R08 (0-100 µM) dose-dependently reduces the level of extracellular HBeAg from HepDES19 cells, with IC₅₀ of ~0.1 µM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	HepDES19 cells
Concentration:	0.3, 1.0, 3.2, 10, 32 µM
Incubation Time:	5 days
Result:	Reduced the level of cccDNA, protein-free RC-DNA, and double stranded linear DNA (DL-DNA).

体内研究
(In Vivo)

ccc_R08 (20 mg/kg; p.o.; twice per day for 2 weeks) clears cccDNA from the liver of HBVcircle mice.
ccc_R08 (10, 15, 20, 30 mg/kg; p.o.; twice per day for 2 weeks) significantly decreases the serum level of pgRNA in a dose-dependent manner, and the reduction in pgRNA is quantitatively correlated with that in liver cccDNA at different doses.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	HBVcircle mouse model .
Dosage:	20 mg/kg
Administration:	Oral administration; twice per day for 2 weeks
Result:	Led to the clearance of cccDNA from HBVcircle mouse livers.

Animal Model:	HBVcircle mouse model .
Dosage:	10, 15, 20, 30 mg/kg
Administration:	Oral administration; twice per day for 42 days
Result:	Led to a sustained reduction in the serum levels of pgRNA.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	HBVcircle mouse model .
Dosage:	20 mg/kg
Administration:	Oral administration; twice per day for 2 weeks
Result:	Led to the clearance of cccDNA from HBVcircle mouse livers.

Animal Model:	HBVcircle mouse model .
Dosage:	10, 15, 20, 30 mg/kg
Administration:	Oral administration; twice per day for 42 days
Result:	Led to a sustained reduction in the serum levels of pgRNA.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	HBVcircle mouse model .
Dosage:	20 mg/kg
Administration:	Oral administration; twice per day for 2 weeks
Result:	Led to the clearance of cccDNA from HBVcircle mouse livers.

Animal Model:	HBVcircle mouse model .
Dosage:	10, 15, 20, 30 mg/kg
Administration:	Oral administration; twice per day for 42 days
Result:	Led to a sustained reduction in the serum levels of pgRNA.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (241.06 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4106 mL	12.0528 mL	24.1057 mL
5 mM	0.4821 mL	2.4106 mL	4.8211 mL
10 mM	0.2411 mL	1.2053 mL	2.4106 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 5 mg/mL (12.05 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (12.05 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 5 mg/mL (12.05 mM); Suspended solution; Need ultrasonic

此方案可获得 5 mg/mL (12.05 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 5 mg/mL (12.05 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (12.05 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。