YS-49 monohydrate|西域试剂

YS-49 monohydrate,99.56%

产品编号：Bellancom-15477A| 分子式：C₂₀H₂₂BrNO₃| 分子量：404.30

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-15477A		￥1228.00	杭州北京（现货）
Bellancom-15477A		￥4315.00	杭州北京（现货）

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YS-49 monohydrate

产品介绍

YS-49 (monohydrate) 是 PI3K/Akt (RhoA 的下游靶标) 的激活剂，可减少 3-甲基胆碱处理的细胞中 RhoA/PTEN 的激活。YS-49 能通过诱导血红素加氧酶 (HO-1) 来抑制血管紧张素 II (Ang II) 刺激 VSMC 细胞的增殖。YS-49 是异喹啉化合物生物碱，因能激活心脏 β-adrenoceptors 而具有强烈的正性肌力作用。

生物活性

YS-49 (monohydrate) is a PI3K/Akt (a downstream target of RhoA) activator, to reduce RhoA/PTEN activation in the 3-methylcholanthrene-treated cells. YS-49 inhibits angiotensin II (Ang II)-stimulated proliferation of VSMCs via induction of heme oxygenase (HO)-1. YS-49 is also an isoquinoline compound alkaloid, has a strong positive inotropic action through activation of cardiac β-adrenoceptors.

体外研究

YS-49 (1-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC₅₀ values of 22 μM and 30 μM, respectively.
YS-49 (10-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	RAVSMC and RAW 264.7 cells
Concentration:	10 μΜ, 30 μΜ and 100 μΜ(RAVSMC); 1 μΜ, 10 μΜ and 100 μM (RAW 264.7)
Incubation Time:	18 hours
Result:	Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, with IC₅₀ values of 22 and 30 μM, respectively.

Western Blot Analysis

Cell Line:	RAVSMC and RAW 264.7 cells
Concentration:	10 μΜ, 30 μΜ and 100 μΜ
Incubation Time:	18 hours
Result:	Concentration-dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ.

体内研究
(In Vivo)

YS-49 (5 mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague Dawley rats (250-300 g)
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 8 hours
Result:	Serum NOx levels were significantly reduced.

体内研究

YS-49 (5 mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague Dawley rats (250-300 g)
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 8 hours
Result:	Serum NOx levels were significantly reduced.

体内研究

YS-49 (5 mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague Dawley rats (250-300 g)
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 8 hours
Result:	Serum NOx levels were significantly reduced.