Endothelin-1 (1-31) (Human) TFA|西域试剂

Endothelin-1 (1-31) (Human) TFA

产品编号：Bellancom-P4159A| 分子式：C₁₆₄H₂₃₇F₃N₃₈O₄₉S₅| 分子量：3742.18

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货号	价格	库存与货期
Bellancom-P4159A	￥2300.00	杭州北京（现货）
Bellancom-P4159A	￥7000.00	杭州北京（现货）
Bellancom-P4159A	￥10500.00	杭州北京（现货）

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Endothelin-1 (1-31) (Human) TFA

产品介绍

Endothelin-1 (1-31) (Human) TFA 是一种有效的血管收缩剂和血压升高剂。Endothelin-1 (1-31) (Human) TFA 来源于 chymase 对大 ET-1 的选择性水解。

生物活性

Endothelin-1 (1-31) (Human) TFA is a potent vasoconstrictor and hypertensive agent. Endothelin-1 (1-31) (Human) TFA is derived from the selective hydrolysis of big ET-1 by chymase.

体外研究

Endothelin-1 (1-31) (Human) (100 pM-100 nM; 24 h) TFA 诱导人系膜细胞增殖。
Endothelin-1 (1-31) (Human) (100 nM; 0-10 min) TFA 在人系膜细胞中诱导 ERK 激活。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	Human mesangial cells
Concentration:	100 pM-100 nM
Incubation Time:	24 h
Result:	Caused an increase in [³H]-thymidine incorporation into the cells in a concentration-dependent manner.

Western Blot Analysis

Cell Line:	Human mesangial cells
Concentration:	100 nM
Incubation Time:	0, 5, 10, 15 and 30 min
Result:	ERK activities rapidly increased 2.45-fold at 5 min and peaked at 10 min. The activities of both ERKs rapidly declined, returning to the baseline control value 30 min after stimulation.

体内研究
(In Vivo)

ET-1 (1-31) (100 nM; single dose) TFA 诱导小鼠肠系膜动脉收缩。收缩可能是由 ET_A 受体介导的，并可能随着年龄的增长而增加。在目前的慢性糖尿病的情况下，男性和女性之间存在明显差异。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICR mice, Streptozocin (HY-13753)-induced diabetic model
Dosage:	100 nM
Administration:	In the organ bath, single dose
Result:	In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ET_A receptor inhibitor.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICR mice, Streptozocin (HY-13753)-induced diabetic model
Dosage:	100 nM
Administration:	In the organ bath, single dose
Result:	In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ET_A receptor inhibitor.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICR mice, Streptozocin (HY-13753)-induced diabetic model
Dosage:	100 nM
Administration:	In the organ bath, single dose
Result:	In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ET_A receptor inhibitor.