RI(dl)-2 TFA is a potent and selective RAD51-mediated D-loop formation inhibitor with an IC₅₀ of 11.1 μM. RI(dl)-2 TFA does not influence RAD51 binding to ssDNA and inhibits homologous recombination (HR) activity in human cells (IC₅₀ of 3.0 μM).

RI(dl)-2 stabilizes nucleoprotein filaments in a nonfunctional state, which are incapable of D-loop activity and simultaneously shielded from related (e.g., RAD52-mediated) pathways that promote single-strand annealing (SSA).
RI(dl)-2 does not modulate the affinity of RAD51 to ssDNA or the stability of preformed RAD51-ssDNA complexes when challenged with high concentrations of salt.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Wei Lv, et al. Development of Small Molecules that Specifically Inhibit the D-loop Activity of RAD51. J Med Chem. 2016 May 26;59(10):4511-25.  [Content Brief]