6-氯-3-吲哚-β-D-半乳糖皮蒽（约含13%乙酸乙酯）[用于生化研究] |6-Chloro-3-indolyl-ß-D-galactopyranoside |cas:138182-21-5|西域试剂

6-氯-3-吲哚-β-D-半乳糖皮蒽（约含13%乙酸乙酯）[用于生化研究] ,6-Chloro-3-indolyl-ß-D-galactopyranoside

产品编号：SIGMA-RES1364C| CAS NO：138182-21-5| 分子式：C14H16ClNO6| 分子量：329.7329

玫瑰-β-D-Gal是一种荧光染料，也是一种β-半乳糖苷酶底物。玫瑰-β-D-Gal在反应后产生粉红色/洋红色，并已用于检测β-Gal活性。

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货号	包装	价格	库存与货期	购买量	操作
SIGMA-RES1364C		￥2531.00	杭州北京（现货）

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产品名称	6-氯-3-吲哚-β-D-半乳糖皮蒽（约含13%乙酸乙酯）[用于生化研究]
英文名称	6-Chloro-3-indolyl-ß-D-galactopyranoside
CAS编号	138182-21-5
产品描述	玫瑰-β-D-Gal是一种荧光染料，也是一种β-半乳糖苷酶底物。玫瑰-β-D-Gal在反应后产生粉红色/洋红色，并已用于检测β-Gal活性。
产品沸点	630.2±55.0 °C at 760 mmHg
产品密度	1.6±0.1 g/cm3
产品闪点	335.0±31.5 °C
精确质量	329.066620
PSA	115.17000
LogP	-0.15
外观性状	固体;White to Light yellow powder to crystal
蒸气压	0.0±1.9 mmHg at 25°C
折射率	1.717
体外研究	指南（以下是我们推荐的方案。该方案仅提供指南，应根据您的具体需要进行修改）。组织免疫染色：1.在二甲基亚砜（DMSO）中制备25 mg/mL Rose-β-D-Gal储备溶液，并储存在-20°C下。保护储备溶液免受光照是至关重要的。2.准备玫瑰-β-D-Gal染色用冲洗缓冲液：0.1%脱氧胆酸钠，0.2%IGEPAL CA-630，2 mM MgCl2，置于0.1 M磷酸钠缓冲液（pH 7.3）中。通过与158mL 1M NaH2PO4、342mL 1M Na2HPO4和500mL水混合，制备1000mL 0.5M磷酸钠缓冲液（pH 7.3）。3.为新鲜玫瑰-β-D-Gal制备底物溶液，在冲洗缓冲液中含有1 mg/mL玫瑰-β-D-Gal、5 mM铁氰化钾和5 mM亚铁氰化钠。4.在PBS中对冷冻组织切片进行复水：用PBS快速冲洗两次，用冲洗缓冲液冲洗10分钟3次。5.暴露于β-半乳糖苷酶底物Rose-β-D-Gal：在37℃下染色，需要多长时间才能看到染色，长达一整夜，在显色过程中保持遮盖和黑暗。用PBS清洗5分钟两次。6.将组织固定在4%PFA中，除非打算继续原位杂交，并继续执行步骤4。注意：Rose-β-D-Gal在醇或有机溶剂中不太稳定，因此必须使用水基复染剂，如Gill’s苏木精，并用水性贴装剂盖住滑动。
稳定性	避免强氧化剂
储存条件	密封、在2 ºC -8 ºC下保存

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危害码 (欧洲)	Xi
风险声明 (欧洲)	36/37/38
安全声明 (欧洲)	S24/25-S36-S26
WGK德国	3

6-Chloro-3-indolyl β-D-Galactopyranoside [for Revision number: 5
Biochemical Research]
SAFETY DATA SHEET

Section1. IDENTIFICATION
6-Chloro-3-indolyl β-D-Galactopyranoside [for Biochemical Research]
Product name:

Revision number:5

Section2. HAZARDS IDENTIFICATION
GHS classification
Not classified
PHYSICAL HAZARDS
HEALTH HAZARDSNot classified
Not classified
ENVIRONMENTAL HAZARDS
GHS label elements, including precautionary statements
None
Pictograms or hazard symbols
Signal wordNo signal word
None
Hazard statements
Precautionary statements:None

Section3. COMPOSITION/INFORMATION ON INGREDIENTS
Substance/mixture:Substance
Components:6-Chloro-3-indolyl β-D-Galactopyranoside [for Biochemical Research]
Percent:>98.0%(LC)
CAS Number:138182-21-5
Synonyms:Salmon-Gal , Rose-Gal
C14H16ClNO6
Chemical Formula:

Section4. FIRST AID MEASURES
Inhalation:Remove victim to fresh air and keep at rest in a position comfortable for breathing.
Get medical advice/attention if you feel unwell.
Skin contact:Remove/Take off immediately all contaminated clothing. Rinse skin with
water/shower. If skin irritation or rash occurs: Get medical advice/attention.
Eye contact:Rinse cautiously with water for several minutes. Remove contact lenses, if present
and easy to do. Continue rinsing. If eye irritation persists: Get medical
advice/attention.
Ingestion:Get medical advice/attention if you feel unwell. Rinse mouth.
A rescuer should wear personal protective equipment, such as rubber gloves and air-
Protection of first-aiders:
tight goggles.

Section5. FIRE-FIGHTING MEASURES
Suitable extinguishingDry chemical, foam, water spray, carbon dioxide.
media:
Specific hazards arising Take care as it may decompose upon combustion or in high temperatures to
from the chemical:generate poisonous fume.
Biochemical Research]

Section5. FIRE-FIGHTING MEASURES
Precautions for firefighters: Fire-extinguishing work is done from the windward and the suitable fire-extinguishing
method according to the surrounding situation is used. Uninvolved persons should
evacuate to a safe place. In case of fire in the surroundings: Remove movable
containers if safe to do so.
When extinguishing fire, be sure to wear personal protective equipment.
Special protective
equipment for firefighters:

Section6. ACCIDENTAL RELEASE MEASURES
Personal precautions,Use personal protective equipment. Keep people away from and upwind of spill/leak.
protective equipment and Entry to non-involved personnel should be controlled around the leakage area by
emergency procedures: roping off, etc.
Environmental precautions: Prevent product from entering drains.
Methods and materials for Sweep dust to collect it into an airtight container, taking care not to disperse it.
containment and cleaning Adhered or collected material should be promptly disposed of, in accordance with
up: appropriate laws and regulations.

Section7. HANDLING AND STORAGE
Precautions for safe handling
Technical measures:Handling is performed in a well ventilated place. Wear suitable protective equipment.
Prevent dispersion of dust. Wash hands and face thoroughly after handling.
Use a local exhaust if dust or aerosol will be generated.
Advice on safe handling: Avoid contact with skin, eyes and clothing.
Conditions for safe storage, including any
incompatibilities
Storage conditions:Keep container tightly closed. Store in a freezer.
Store under inert gas.
Store away from incompatible materials such as oxidizing agents.
Heat-sensitive, Light-sensitive, Air-sensitive
Packaging material:Comply with laws.

Section8. EXPOSURE CONTROLS / PERSONAL PROTECTION
Engineering controls:Install a closed system or local exhaust as possible so that workers should not be
exposed directly. Also install safety shower and eye bath.
Personal protective equipment
Respiratory protection: Dust respirator. Follow local and national regulations.
Hand protection:Protective gloves.
Eye protection:Safety glasses. A face-shield, if the situation requires.
Skin and body protection: Protective clothing. Protective boots, if the situation requires.

Section9. PHYSICAL AND CHEMICAL PROPERTIES
Solid
Physical state (20°C):
Form:Crystal- Powder
Colour:White - Slightly pale yellow
Odour:No data available
pH: No data available
Melting point/freezing point:No data available
Boiling point/range:No data available
No data available
Flash point:
Flammability or explosive
limits:
Lower:No data available
No data available
Upper:
Relative density:No data available
Solubility(ies):
[Water]No data available
[Other solvents]No data available
Biochemical Research]

Section10. STABILITY AND REACTIVITY
Chemical stability:Stable under proper conditions.
Possibility of hazardous No special reactivity has been reported.
reactions:
Incompatible materials: Oxidizing agents
Hazardous decomposition Carbon monoxide, Carbon dioxide, Nitrogen oxides (NOx), Hydrogen chloride
products:

Section11. TOXICOLOGICAL INFORMATION
Acute Toxicity:No data available
Skin corrosion/irritation: No data available
Serious eyeNo data available
damage/irritation:
Germ cell mutagenicity: No data available
Carcinogenicity:
IARC =No data available
NTP =No data available
Reproductive toxicity:No data available

Section12. ECOLOGICAL INFORMATION
Ecotoxicity:
No data available
Fish:
Crustacea:No data available
No data available
Algae:
Persistence / degradability: No data available
No data available
Bioaccumulative
potential(BCF):
Mobility in soil
Log Pow:No data available
No data available
Soil adsorption (Koc):
Henry's LawNo data available
constant(PaM3/mol):

Section13. DISPOSAL CONSIDERATIONS
Recycle to process, if possible. Consult your local regional authorities. You may be able to dissolve or mix material
with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber system.
Observe all federal, state and local regulations when disposing of the substance.

Section14. TRANSPORT INFORMATION
Hazards Class:Does not correspond to the classification standard of the United Nations
Not listed
UN-No:

Section15. REGULATORY INFORMATION
Safe management ordinance of dangerous chemical product (State Council announces on January 26, 2002
and revised on February 16,2011): Safe use and production, the storage of a dangerous chemical, transport,
loading and unloading were prescribed.
Biochemical Research]

SECTION 16 - ADDITIONAL INFORMATION
N/A

[1]. Ismail J A , et al. Immunohistologic labeling of murine endothelium[J]. 2003, 12(2):0-90.

[2]. Komatsu Y, et al. In situ hybridization methods for mouse whole mounts and tissue sections with and without additional β-galactosidase staining. Methods Mol Biol. 2014;1092:1-15.