黄豆苷原|Daidzein|cas:486-66-8|西域试剂

黄豆苷原,Daidzein,≥98%

产品编号：SIGMA-D7802| CAS NO：486-66-8| 分子式：C15H10O4| 分子量：254.2375

Daidzein (黄豆苷元) 是genistein的钝化类似物，是酪氨酸激酶抑制剂和雌激素受体活化剂。Daidzein可以抑制TNF-α诱导的Cxcl2表达和NF-κB转录活性的增加，同时也可以降低PARP-1活性。

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	包装	价格	库存与货期	购买量	操作
SIGMA-D7802		￥1413.00	杭州北京（现货）
SIGMA-D7802		￥4629.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

产品名称

黄豆苷原

英文名称

Daidzein

CAS编号

486-66-8

产品描述

产品熔点

315-323°C (dec.)

产品沸点

512.8±50.0 °C at 760 mmHg

产品密度

1.4±0.1 g/cm3

产品闪点

201.2±23.6 °C

精确质量

254.057907

PSA

70.67000

LogP

2.78

外观性状

白色至灰白色结晶粉末

蒸气压

0.0±1.4 mmHg at 25°C

折射率

1.699

溶解性

溶于乙醇及乙醚。

溶解性数据

In Vitro:

DMSO : ≥ 50 mg/mL (196.66 mM)

H₂O : < 0.1 mg/mL (ultrasonic) (insoluble)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.9333 mL	19.6665 mL	39.3329 mL
5 mM	0.7867 mL	3.9333 mL	7.8666 mL
10 mM	0.3933 mL	1.9666 mL	3.9333 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 50% PEG300 50% saline
Solubility: 20 mg/mL (78.67 mM); Suspended solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (9.83 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.83 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.83 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.83 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

靶点

PPAR-α

PPAR-γ

体外研究

在3T3-L1脂肪细胞中，大豆苷元通过共培养逆转脂联素基因表达的减弱，并且这些作用被PPAR-γ特异性抑制剂抑制。大豆苷元减弱脂肪细胞中脂联素表达的减少，并且PPAR-γ特异性抑制剂消除了这种作用。通过荧光素酶报道分子测定证实大豆苷元直接激活PPAR-α和γ。在HEK293T细胞中，大豆苷元以浓度依赖性方式显着增加PPAR-α转录活性。尽管在PPAR-γ转录活性中未观察到明显的剂量依赖性，但大豆苷元在大豆苷元增强PPAR-α转录活性的相似浓度范围内也显着增加PPAR-γ转录活性，最大增加为25μM ]。大豆苷元是大豆异黄酮，其上调Abcg1的表达，并且通过雌激素受体信号传导促进培养的海马神经元中的轴突生长。大豆苷元是大豆的主要成分，与雌激素具有结构相似性。它发挥抗炎作用，降低脂质水平，并增加线粒体生物发生。作为核受体过氧化物酶体增殖物激活受体（PPARs）的激活剂，大豆苷元增强PPARs依赖性基因的转录，包括肝X受体（LXRs，小鼠中的Nr1h基因家族）。用不同浓度的大豆黄酮孵育，从5到100μM，增加APOE转录活性。

体内研究

用大豆黄酮处理Apoe KO小鼠在中风后1个月增加Lxr和Abca1基因表达，表明缺乏ApoE不会干扰其他胆固醇稳态遗传程序。因此，研究结果表明，大豆苷元诱导的ApoE上调是促进慢性卒中功能恢复的关键因素。

细胞实验

将HEK293T细胞以约2.5×10 4个细胞/孔的细胞密度接种在24孔板上，并生长至70-80％汇合。然后使用PP-α或PPAR-γ表达质粒瞬时转染细胞，并使用X-treme GENE HP DNA转染在含有三个串联PPAR反应元件（PPRE×3 TK-荧光素酶）的控制下的荧光素酶基因质粒转染。试剂。将海肾荧光素酶对照载体共转染以控制转染效率。转染后，将细胞在含有DMSO或各种浓度（6.25,12.5,25μM）大豆苷元的培养基中再培养24小时。裂解细胞，测量荧光素酶活性并表示为诱导倍数，将其标准化为海肾荧光素酶对照质粒的活性。

动物实验

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.9333 mL	19.6665 mL	39.3329 mL
5 mM	0.7867 mL	3.9333 mL	7.8666 mL
10 mM	0.3933 mL	1.9666 mL	3.9333 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m²)	0.007	0.025	0.15	0.05	0.02	0.5
K_m 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

稳定性

按规格使用和贮存，不会发生分解，避免与氧化物接触

储存条件

粉末型式 -20°C 3年；4°C 2年

溶于溶剂 -80°C 6个月；-20°C 1个月

运输方式冰袋运输，根据产品的不同，可能会有相应调整。

化学品安全说明书(MSDS)

下载MSDS

质检证书(COA)

符号	GHS07
信号词	Warning
危害声明	H315-H319
警示性声明	P280-P305 + P351 + P338-P337 + P313
个人防护装备	dust mask type N95 (US);Eyeshields;Gloves
危害码 (欧洲)	Xi:Irritant;
风险声明 (欧洲)	R36/38
安全声明 (欧洲)	S24-S26-S37/39
危险品运输编码	NONH for all modes of transport
WGK德国	3
RTECS号	DJ3100040

1. 物质的识别

产品名：	Daidzein
CAS号：	486-66-8
制造商/供应商：	西域试剂网站：www.hzbp.cn 邮件：13911702513@139.com

2. 合成/成分数据

产品名：	Daidzein
别名：	Isoflavone
分子式：	C15H10O4
分子量：	254.24

3. 急救措施

吸入后：	如果吸入，移至空气新鲜处，如果呼吸困难，给输氧，如呼吸停止，给予人工呼吸。
皮肤接触后：	用大量的水冲洗，移除污染的衣服和鞋子。
眼睛接触后：	检查并取下隐形眼镜，并用大量的水冲洗；呼叫医生。
吞食后：	如果吞食，用大量纯净水漱口；呼叫医生。

4. 消防措施

适当的灭火剂：	雾状水，二氧化碳，干粉或泡沫。
防护设备：	穿戴自给式呼吸器和防护服，以防止与皮肤和眼睛接触。

5. 泄漏应急处理

安全防范措施：	封锁泄漏区域；穿戴自给式呼吸器，防护服和厚橡胶手套。
清洁/收集措施：	使用液体粘合原料（硅藻土，通用粘合剂）吸取精细粉末；使用酒精擦洗表面和设备除去污渍；根据第11条处理被污染的材料。

6. 处理和储存

安全处理说明：	避免吸入和接触皮肤，眼睛及衣物；材料可能略微具有刺激性。
储存：	粉末型式 -20°C 3年；4°C 2年溶于溶剂 -80°C 6个月；-20°C 1个月

7. 接触控制和个人防护

呼吸设备：	NIOSH / MSHA认可的呼吸器。
双手保护：	耐化学腐蚀的橡胶手套。
眼睛防护：	化学安全护目镜。

8. 稳定性和反应活性

稳定性：	按照说明存储是稳定的；避免强氧化剂。
热分解/其他要避免的情况：	避免光和热。

9. 毒性资料

急性毒性：	无可用资料。
主要刺激性影响：	无可用资料。
在皮肤上：	无可用资料。
对眼睛：	无可用资料；可能具有刺激性。

10. 生态资料

一般注意事项：

无可用资料。

11. 废弃处置

按照所在国家，省份，县市和地方的法规处置。

12. 运输信息

正确的运输名称：	无
非危险品运输：	这种物质被视为非危险品运输。

13. 法规信息

尚未有针对此产品作出的化学安全性评估。

14. 其他信息

上游产品 9
485-72-3 2491-38-5 95-01-2 56401-06-0
17720-60-4 122-51-0 68-12-2 552-66-9
156-38-7
下游产品 10
17817-31-1 40957-83-3 485-63-2 485-72-3
1157-39-7 486-63-5 21255-69-6 291759-05-2
552-66-9 602329-51-1

94. [2.602] LIU Xu et al."Quality assessment of fermented soybeans: physicochemical, bioactive compounds and biogenic amines."Food Science and Technology.2023 Mar;43:e102722

93. [5.123] Jian Wang et al."Solid-State Fermentation of Soybean Meal with Edible Mushroom Mycelium to Improve Its Nutritional, Antioxidant Capacities and Physicochemical Properties."Fermentation-Basel.2023 Apr;9(4):322

92. [5.561] Yuan Gao et al."Determination of Isoflavones in Radix puerariae from Different Origins by Ultra-High Performance Liquid Chromatography Based on Optimal Pretreatment Method."Foods.2023 Jan;12(4):794

91. [6.656] Yanchao Xing et al."Cytochrome P450-mediated herb-drug interaction (HDI) of Polygonum multiflorum Thunb. based on pharmacokinetic studies and in vitro inhibition assays."PHYTOMEDICINE.2023 Feb;:154710

90. [5.911] Yuting Chen et al."Effects of selected Bacillus strains on the biogenic amines, bioactive ingredients and antioxidant capacity of shuidouchi."INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY.2023 Mar;388:110084

89. [IF=7.675] Yongzhu Zhang et al."Soy Isoflavones Protect Neuronal PC12 Cells against Hypoxic Damage through Nrf2 Activation and Suppression of p38 MAPK and AKT–mTOR Pathways."Antioxidants.2022 Oct;11(10):2037

88. [IF=6.073] Huang Lili et al."Soybean isoflavones modulate gut microbiota to benefit the health weight and metabolism."Frontiers in Cellular and Infection Microbiology.2022 Sep;0:1288

87. [IF=6.208] Pan Wang et al."Discovery of Flavonoids as Novel Inhibitors of ATP Citrate Lyase: Structure–Activity Relationship and Inhibition Profiles."INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.2022 Jan;23(18):10747

86. [IF=3.571] Gen Xue et al."The qualitative and quantitative profiling for quality assessment of Yinxing Mihuan Oral Solution and the stability study on the focused flavonol glycosides."JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS.2022 Sep;219:114937

85. [IF=5.561] Aoli Xiang et al."Determination of 14 Isoflavone Isomers in Natto by UPLC-ESI-MS/MS and Antioxidation and Antiglycation Profiles."Foods.2022 Jan;11(15):2229

84. [IF=7.514] Rui Wang et al."Functionalization of soy residue (okara) by enzymatic hydrolysis and LAB fermentation for B2 bio-enrichment and improved in vitro digestion."Food Chem. 2022 Sep;387:132947

83. [IF=6.576] Junkun Pan et al."Inhibition of Dipeptidyl Peptidase-4 by Flavonoids: Structure–Activity Relationship, Kinetics and Interaction Mechanism."Frontiers in Nutrition. 2022; 9: 892426

82. [IF=7.514] Jie Meng et al."Conduction of a chemical structure-guided metabolic phenotype analysis method targeting phenylpropane pathway via LC-MS: Ginkgo biloba and soybean as examples."FOOD CHEMISTRY. 2022 Oct;390:133155

81. [IF=11.614] Pengfei He et al."Structural mechanism of a dual-functional enzyme DgpA/B/C as both a C-glycoside cleaving enzyme and an O- to C-glycoside isomerase."Acta Pharmaceutica Sinica B. 2022 May;:

80. [IF=7.514] Xuefeng Chen et al."Quantitative analyses for several nutrients and volatile components during fermentation of soybean by Bacillus subtilis natto."Food Chem. 2021 Dec;:131725

79. [IF=6.475] Pin Chen et al."The bioavailability of soy isoflavones in vitro and their effects on gut microbiota in the simulator of the human intestinal microbial ecosystem."Food Res Int. 2021 Dec;:110868

78. [IF=7.514] Chengwen Lu et al."Effect of pulsed electric field on soybean isoflavone glycosides hydrolysis by β-glucosidase: Investigation on enzyme characteristics and assisted reaction."Food Chem. 2022 Jun;378:132032

77. [IF=3.645] Jingbin Chen et al.Screening for potential quality markers of Callerya nitida var. hirsutissima. Z.Wei based on components profile, pharmacokinetics, and anti-inflammatory study.Journal Of Separation Science.2021 Nov 02

76. [IF=1.797] Bihui Liu et al."Effects of Lactobacillus plantarum CQPC01‐fermented soybean milk on activated carbon‐induced constipation through its antioxidant activity in mice."Food Sci Nutr. 2019 Jun;7(6):2068-2082

75. [IF=2.742] Guo Yingyu et al."Isolation and identification of a human intestinal bacterium capable of daidzein conversion."Fems Microbiol Lett. 2021 May;368(8):

74. [IF=2.984] Chenkai Wang et al."Comparative transcriptome analysis of roots, stems, and leaves of Pueraria lobata (Willd.) Ohwi: identification of genes involved in isoflavonoid biosynthesis."Peerj. 2021 Feb;9:e10885

73. [IF=3] Qu Lala et al."Phenotypic assessment and ligand screening of ETA/ETB receptors with label-free dynamic mass redistribution assay."N-S Arch Pharmacol. 2020 Jun;393(6):937-950

72. [IF=3] Run-Jia XU et al."3′-Methoxydaidzein exerts analgesic activity by inhibiting voltage-gated sodium channels."Chin J Nat Medicines. 2019 Jun;17:413

71. [IF=3.361] Mengmeng Yuan et al."The interaction of dietary flavonoids with xanthine oxidase in vitro: molecular property-binding affinity relationship aspects."Rsc Adv. 2019 Apr;9(19):10781-10788

70. [IF=3.373] Wanning Chen et al."The composition differences between small black beans and big black beans from different habitats and its effects on the processing of Polygonum multiflorum."Phytochem Analysis. 2021 Sep;32(5):767-779

69. [IF=3.422] Wei Bin et al."Discovery of novel glycoside hydrolases from C-glycoside-degrading bacteria using sequence similarity network analysis."J Microbiol. 2021 Oct;59(10):931-940

68. [IF=3.512] Yan Cheng et al."Comparison of the Extraction Efficiency of Isoflavone Compounds from Puerariae lobatae by Ionic Liquids with 11 Anions and 8 Imidazolium-Based Cations."Acs Omega. 2020;5(15):8962–8971

67. [IF=3.645] Hongmin Zhang et al."Simultaneous determination of five isoflavones in rat plasma by LC-MS/MS: Comparative pharmacokinetic characteristics of Puerariae lobatae radix in normal and type 2 diabetic rats."J Sep Sci. 2019 Aug;42(16):2592-2601

66. [IF=3.935] Zhengchao Ji et al."Global identification and quantitative analysis of representative components of Xin-Nao-Kang Capsule, a traditional Chinese medicinal formula, by UHPLC-Q-TOF-MS and UHPLC-TQ-MS."J Pharmaceut Biomed. 2021 May;198:114002

65. [IF=3.935] Yuqi Mei et al."Qualitative and quantitative analysis of the major constituents in Spatholobi Caulis by UFLC-Triple TOF-MS/MS and UFLC-QTRAP-MS/MS."J Pharmaceut Biomed. 2021 Feb;194:113803

64. [IF=3.935] Yanchao Xing et al."An effective strategy for distinguishing the processing degree of Polygonum multiflorum based on the analysis of substance and taste by LC-MS, ICP-OES and electronic tongue."J Pharmaceut Biomed. 2021 Oct;205:114328

63. [IF=4.411] Liyu Luo et al."Integrated Phytochemical Analysis Based on UPLC-MS and Network Pharmacology Approaches to Explore the Quality Control Markers for the Quality Assessment of Trifolium pratense L.."Molecules. 2020 Jan;25(17):3787

62. [IF=4.411] Liqing Yin et al."Neuroprotective Potency of Tofu Bio-Processed Using Actinomucor elegans against Hypoxic Injury Induced by Cobalt Chloride in PC12 Cells."Molecules. 2021 Jan;26(10):2983

61. [IF=4.411] Yuqi Mei et al."A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis."Molecules. 2020 Jan;25(1):167

60. [IF=4.703] Wang Qiao et al."Preparation and Pharmacokinetic Study of Daidzein Long-Circulating Liposomes."Nanoscale Res Lett. 2019 Dec;14(1):1-10

59. [IF=4.759] Wenjie Wu et al."An analytical strategy for accurate, rapid and sensitive quantitative analysis of isoflavones in traditional Chinese medicines using ultra-high performance supercritical fluid chromatography: Take Radix Puerariae as an example."J Chromato

58. [IF=4.879] Yang Liu et al."UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings."Biomolecules. 2020 Apr;10(4):504

57. [IF=5.64] Liang Wenou et al."Colonization Potential to Reconstitute a Microbe Community in Pseudo Germ-Free Mice After Fecal Microbe Transplant From Equol Producer."Front Microbiol. 2020 Jun;0:1221

56. [IF=5.81] Han Lifeng et al."Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis."Front Pharmacol. 2019 Apr;0:329

55. [IF=7.132] Li Yang et al."Supercritical extraction and antioxidant activity of major ingredients in Puerariae lobatae root, Pinus massoniana needle, Citrus reticulata peel and their mixture."J Co2 Util. 2021 Jun;48:101518

54. [IF=7.491] Guoping Yu et al."Preparation of Daidzein microparticles through liquid antisolvent precipitation under ultrasonication."Ultrason Sonochem. 2021 Nov;79:105772

53. [IF=7.514] Xiaoming Yu et al."Impact of processing technologies on isoflavones, phenolic acids, and antioxidant capacities of soymilk prepared from 15 soybean varieties."Food Chem. 2021 May;345:128612

52. [IF=1.913] Zhu Yi-Lin et al."Composition, Distribution, and Antioxidant Activity of Phenolic Compounds in 18 Soybean Cultivars."J Aoac Int. 2018 Mar;101(2):520-528

51. [IF=2.19] Y.J. Chen et al."Phytochemical Profiles of Edible Kudzu (Pueraria thomsonii Benth) Grown in China as Affected by Thermal Processing."J Food Process Pres. 2017 Feb;41(1):e12754

50. [IF=2.896] Bo Li et al."Preparative separation of isoflavones in plant extract of Pueraria lobata by high performance counter-current chromatography."Anal Methods-Uk. 2015 Feb;7(4):1321-1327

49. [IF=3.361] Hongguang Zhang et al."Extraction of isoflavones from Puerariae lobata using subcritical water."Rsc Adv. 2018 Jun;8(40):22652-22658

48. [IF=3.645] Wenjie Wu et al."Simple, rapid, and environmentally friendly method for the separation of isoflavones using ultra-high performance supercritical fluid chromatography."J Sep Sci. 2017 Jul;40(13):2827-2837

47. [IF=3.738] Selma Houchi et al."Investigation of common chemical components and inhibitory effect on GES-type β-lactamase (GES22) in methanolic extracts of Algerian seaweeds."Microb Pathogenesis. 2019 Jan;126:56

46. [IF=4.411] Yan-Fang Yang et al."Distribution Assessments of Coumarins from Angelicae Pubescentis Radix in Rat Cerebrospinal Fluid and Brain by Liquid Chromatography Tandem Mass Spectrometry Analysis."Molecules. 2018 Jan;23(1):225

45. [IF=4.952] Guocheng Huang et al."Improvement in beta-carotene, vitamin B2, GABA, free amino acids and isoflavones in yellow and black soybeans upon germination."Lwt Food Sci Technol. 2017 Jan;75:488

44. [IF=7.46] Yiming Liang et al."Molecularly imprinted electrochemical sensor for daidzein recognition and detection based on poly(sodium 4-styrenesulfonate) functionalized graphene."Sensor Actuat B-Chem. 2017 Nov;251:542

43. [IF=7.514] Liang Lv et al."Thermally-induced whey protein isolate-daidzein co-assemblies: Protein-based nanocomplexes as an inhibitor of precipitation/crystallization for hydrophobic drug."Food Chem. 2019 Mar;275:273

42. [IF=7.514] Lu Xu et al."A systematic, comparative study on the beneficial health components and antioxidant activities of commercially fermented soy products marketed in China."Food Chem. 2015 May;174:202

41. [IF=7.514] Xiya Huang et al."Kinetic changes of nutrients and antioxidant capacities of germinated soybean (Glycine max L.) and mung bean (Vigna radiata L.) with germination time."Food Chem. 2014 Jan;143:268

40. Zhou, Jing, et al. "A strategy for rapid discovery of traceable chemical markers in herbal products using MZmine 2 data processing toolbox: A case of Jing Liqueur." Chinese Herbal Medicines (2021).

39. Zhu, Yi-Lin, et al. "Composition, distribution, and antioxidant activity of phenolic compounds in 18 soybean cultivars." Journal of AOAC International 101.2 (2018): 520-528.https://doi.org/10.5740/jaoacint.17-0156

38. Huang, Xiya, Weixi Cai, and Baojun Xu. "Kinetic changes of nutrients and antioxidant capacities of germinated soybean (Glycine max L.) and mung bean (Vigna radiata L.) with germination time." Food chemistry 143 (2014): 268-276.https://doi.org/10.1016/j.foo

37. Zhang, Hongguang, et al. "Extraction of isoflavones from Puerariae lobata using subcritical water." RSC advances 8.40 (2018): 22652-22658.https://doi.org/10.1039/C8RA02653J

36. Zhang, Hongguang, et al. "Extraction of isoflavones from Puerariae lobata using subcritical water." RSC advances 8.40 (2018): 22652-22658.https://doi.org/10.1039/C8RA02653J

35. Huang, Guocheng, Weixi Cai, and Baojun Xu. "Improvement in beta-carotene, vitamin B2, GABA, free amino acids and isoflavones in yellow and black soybeans upon germination." LWT 75 (2017): 488-496.https://doi.org/10.1016/j.lwt.2016.09.029

34. Yiming Liang, Chen Qu, Ran Yang, Lingbo Qu, Jianjun Li, Molecularly imprinted electrochemical sensor for daidzein recognition and detection based on poly(sodium 4-styrenesulfonate) functionalized graphene, Sensors and Actuators B: Chemical, Volume 251, 201

33. Yang, Yan-Fang, Lei Zhang, and Xiu-Wei Yang. "Distribution assessments of coumarins from Angelicae Pubescentis Radix in rat cerebrospinal fluid and brain by Liquid Chromatography Tandem Mass Spectrometry analysis." Molecules 23.1 (2018): 225.https://doi.or

32. 黄玉军,周帆,李颖华,于俊娟,顾瑞霞.植物乳杆菌58发酵豆乳产大豆异黄酮苷元条件的优化[J].现代食品科技,2021,37(02):183-190.

31. 李承浩,王萌,任晓亮.磺化杯6芳烃对4类中药单体成分增溶作用及其机制研究[J].中国现代中药,2020,22(12):2032-2038.

30. 周文红,郭咪咪,毕艳红,王朝宇,段章群.酶解制备苷元型大豆异黄酮[J].中国油脂,2020,45(12):100-104.

29. 王晶,赵重博,唐家琪,卜雕雕,邹俊波,张小飞,程江雪,王昌利.高效液相色谱法同时测定复方龙脉宁中7种成分的含量[J].中南药学,2020,18(11):1903-1905.

28. 常冠华,薄颖异,崔洁,徐露露,赵梓邯,王文全,侯俊玲.基于UPLC-Q-Exactive Orbitrap-MS分析甘草地上部分主要化学成分[J].中国中药杂志,2021,46(06):1449-1459.

27. 许金国,黄紫炎,沈钱能,李林,王巧晗,王璐,陆兔林,毛春芹.经典名方竹茹汤的指纹图谱及功效关联物质预测分析[J].中国中药杂志,2020,45(23):5599-5606.

26. 曹冬英,李鸷,许文,隋利强,徐伟.4种市售黑豆及成品淡豆豉中异黄酮含量分析[J].药学研究,2020,39(10):581-584.

25. 杜静,王琪琪,王云胜,张传博.冠突散囊菌发酵对葛根的活性物质和抗氧化活性的影响[J].食品工业科技,2021,42(01):121-125+131.

24. 梅余琪,魏丽芳,邹立思,刘训红,陈佳丽,谈梦霞,王程成,蔡芷辰,殷圣鑫,张芙蓉.鸡血藤韧皮部环数与多指标成分的相关性分析[J].分析测试学报,2020,39(08):941-949.

23. 黄玉军,周帆,于俊娟,李颖华,顾瑞霞.高转化大豆异黄酮乳酸菌的筛选及在豆乳中的发酵特性[J].食品研究与开发,2021,42(03):157-162.

22. 陈佳丽,谈梦霞,邹立思,刘训红,陈舒妤,石婧婧,王程成,梅余琪.超快速液相色谱-三重四级杆/线性离子阱质谱法同时测定不同产地竹节参药材中多元成分[J].中国药学杂志,2019,54(03):226-233.

21. 周凯文, 陈晓默, 刘慧琳,等. 多酚黄酮物质对晚期糖基化终产物的抑制研究[J]. 食品研究与开发, 2018, v.39;No.329(04):7-13.

20. 赵石磊, 何旭, 王爱珠,等. 酶法提高豆浆中大豆异黄酮苷元含量的工艺研究[J]. 食品工业, 2019, 040(001):100-104.

19. 郭天赐, 赵石磊, 刘石生. 苦杏仁β-葡萄糖苷酶水解豆浆中大豆异黄酮的工艺研究[J]. 食品研究与开发, 2019(12).

18. 唐君, 付强, 崔勐,等. 黄酮与溶菌酶相互作用的强度衰减-基质辅助激光解吸离子化-质谱研究[J]. 分析化学, 2016, 044(007):1071-1076.

17. 靳羽慧, 刘长忠, 徐响,等. 蒸汽爆破对豆渣中大豆异黄酮的影响研究[J]. 中国粮油学报, 2017(10).

16. 刘杰何钢刘贤桂等. 人工栽培鸡血藤中五种黄酮类物质含量测定[J]. 分子植物育种 2017 015(009):3832-3837.

15. 吴文杰邓阳谭桂林罗娟陈秧黄莺张峰.一测多评法测定葛根药材中5种异黄酮类成分[J].中草药 2017 48(04):777-781.

14. 朱怡霖, 张海生, 赵鑫帅,等. 大孔树脂分离纯化横山老黑豆酚类物质[J]. 食品与生物技术学报, 2019, 038(009):103-110.

13. 刘露马金同沈小梅等. 一种保健酒中5种大豆异黄酮及芝麻素的超高效液相色谱检测法[J]. 酿酒 2019 046(004):86-88.

12. 王飞霞杨晓华张华峰朱春燕李璐王凤忠.3种豆芽中异黄酮、多酚的体外抗氧化活性及其对果蝇SOD、GSH-Px活力的影响[J].中国食品学报 2018 18(11):57-64.

11. ]滕飞[1], 李博[1], 谢静[1],等. 葛根中抑制磷酸二酯酶4活性成分的筛选研究[J]. 分子科学学报(中,英文), 2018(1).

10. 申春莉, 沙见宇, 李曼,等. 灵芝固态发酵豆渣的抗氧化特性变化研究[J]. 食品研究与开发, 2019, 040(024):60-64.

9. 李圆圆, 田晨颖, 刘晓美,等. 添加微量元素对黑豆发芽过程中成分的影响[J]. 食品工业科技, 2019, 40(09):104-110.

8. 李博,赵安琦,张慧荣,李丽.应用超滤液相色谱和电喷雾质谱技术筛选MMP-2活性成分[J].分子科学学报,2015,31(02):94-100.

7. 沈发迪, 张震, 梁武,等. 工业化规模生产的含有后生素益生菌纳豆固体饮料的研究[J]. 职业与健康, 2020, v.36(05):43-47.

6. 黄紫炎沈钱能李平等. 不同产地葛根饮片的UPLC指纹图谱结合多成分含量测定研究[J]. 中国中药杂志 2019 v.44(10):93-100.

5. 梅余琪, 魏丽芳, 邹立思,等. 鸡血藤韧皮部环数与多指标成分的相关性分析[J]. 分析测试学报, 2020(8):941-949.

4. 梅余琪, 魏丽芳, 邹立思,等. 鸡血藤中多元活性成分动态积累的分析与评价[J]. 中国中药杂志, 2020, v.45(03):134-145.

3. 张圳, 冯家懿, 果卉,等. 高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物[J]. 药学实践杂志, 2018(4).

2. 刘舵, 杨永利, 雷婷,等. 枸杞子乙酸乙酯提取物急性毒性及对切除卵巢大鼠骨质疏松的防治作用[J]. 中国骨质疏松杂志, 2016, 22(04):396-401.

1. 周荧,魏建林,罗胜保,张欣,彭毛.固定化β-葡萄糖苷酶在异黄酮生物转化中的应用[J].粮食科技与经济,2016,41(02):37-40+50.