Thermolysin（100μg，1 mL，64℃，4小时，在分离的淀粉颗粒中）消化可选择性去除一组介于10和27 kD之间的低分子量蛋白质。热溶素可用于牛肝肌浆蛋白的水解。

Thermolysin（50 mg/kg，口服一次）可降低SHR大鼠的收缩压。热溶素无急性毒性，大鼠口服LD50超过18000 mg/kg，小鼠口服LD50大于24000 mg/kg（急性毒性研究）[5]。动物模型：自发性高血压大鼠（SHR）剂量：50 mg/kg给药：口服（p.o.），一次结果：SHR大鼠收缩压降低，给药后6小时观察到收缩压最大降低22 mm。

Application A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C . Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala ; and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

-20°C密闭，避光，通风干燥处