酰化酶 来源于黑曲霉 ,Aminoacylase

产品编号:SIGMA-01818| CAS NO:9012-37-7| 分子式:C₃₀H₃₄Cl₂N₄O| 分子量:537.523165225983

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货号 包装 价格 库存与货期 购买量 操作
SIGMA-01818
1739.00 杭州 北京(现货)
SIGMA-01818
2303.00 杭州 北京(现货)
SIGMA-01818
1364.00 杭州 北京(现货)
SIGMA-01818
527.00 杭州 北京(现货)
SIGMA-01818
6665.00 杭州 北京(现货)

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产品名称 酰化酶 来源于黑曲霉
英文名称Aminoacylase
CAS编号9012-37-7
产品沸点813.3±65.0 °C at 760 mmHg
产品密度1.3±0.1 g/cm3
产品闪点445.7±34.3 °C
精确质量536.210938
LogP7.64
外观性状固体;White to Amber powder to crystalline
蒸气压0.0±2.9 mmHg at 25°C
折射率1.668
稳定性

Analysis Note Protein determined by biuret.

Unit Definition One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

Biochem/physiol Actions The enzyme hydrolyzes a variety of N-acyl-L-amino acids and dehydropeptides containing dehydroalanyl residues. N-acyl-D-amino acids and dehydropeptides other than dehydroalanine are not hydrolyzed. The following compounds are hydrolyzed by acylase I: chloroacetyl-L-amino acids, L-leucinamide, chloroacetyldehydroalanine, glycyl-L-amino acids, and glycyldehydroalanine. Aminoacylase is widely used as a reagent to resolve amino acid racemates. The Km for acyl amino acids have been reported in the literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), and Ac-L-Phe (5.5 mM).

Aminoacylase from pig kidney is activated by Zn2+ and Co2+ and inhibited by SH blocking reagents, disulfide reducing reagents, N-tosyl-L-alanine,

DL-norleucine, and N-tosyl-L-lysine chloromethylketone.

储存条件

密封-20℃干燥保存。

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