dNTPs,Deoxynucleotide Mix (10 mM, ≥99%)

产品编号:Sigma-D7295| CAS NO:Null| MDL NO:Null,without related properties.| 分子式:Null,without related properties.| 分子量:Null,without related properties.

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货号 包装 价格 库存与货期 购买量 操作
Sigma-D7295
230.00 杭州 北京(现货)

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产品性状

Null,without related properties.

质量标准

PCR Suitability
dNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing10 mM Trizma?-HCl, pH 8.3 at 25 °C, 50 mM KCl, 1.5 mM MgCl2,
0.001% (w/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng/100 μl, and Taq DNA polymerase at 2.5 units/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments, and 72 °C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel.

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