溶菌酶|Lysozyme from chicken egg white|cas:12650-88-3|西域试剂

溶菌酶,Lysozyme from chicken egg white, (≥40KU/mg)

产品编号：Sigma-L6876| CAS NO：12650-88-3| MDL NO：MFCD00131557| 分子式：Null,without related properties.| 分子量：Null,without related properties.

Lysozyme（溶菌酶）是鸡蛋中存在的一种溶菌酶，专门作用于微生物细胞壁的水解酶，又称细胞壁溶解酶。溶菌酶作用底物的特异性很强，不同来源的溶菌酶作用的底物不同。溶菌酶对革兰氏阳性菌、好气性孢子形成菌、枯草杆菌、耐辐射微球菌均有良好的分解作用,对大肠杆菌、普通变球菌和副溶血性弧菌等革兰氏阴性菌也有一定程度的溶解作用。溶菌酶具有分解细菌细胞壁中肽聚糖的特殊作用。主要通过破坏细胞壁中的N-乙酰胞壁酸和N-乙酰氨基葡糖之间的β-1,4糖苷键，使细胞壁不溶性黏多糖分解成可溶性糖肽，导致细胞壁破裂内容物逸出而使细菌溶解。溶菌酶还可与带负电荷的病毒蛋白直接结合，与DNA、RNA、脱辅基蛋白形成复盐，使病毒失活。可分解溶壁微球菌、巨大芽孢杆菌、黄色八叠球菌等革兰阳性菌。

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Sigma-L6876	￥280.00	杭州北京（现货）
Sigma-L6876	￥760.00	杭州北京（现货）
Sigma-L6876	￥3750.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

产品名称

溶菌酶

英文名称

Lysozyme from chicken egg white

CAS编号

12650-88-3

产品描述

外观性状

powder | white

溶解性

Water 10 mg/mL

溶解性数据

In Vitro:

H₂O : 10 mg/mL (Need ultrasonic and warming)

靶点

Bacteria

体外研究

溶菌酶是一种普遍存在的酶。鸡蛋是最丰富的溶菌酶来源，占蛋白质蛋白质的约3.4％。溶菌酶是天然的抗微生物剂，其水解N-乙酰胞壁酸和在细菌细胞壁的肽聚糖层中发现的N-乙酰葡糖胺之间的β（1-4）糖苷键并引起细胞裂解。溶菌酶的杀菌作用主要限于革兰氏阳性细菌，包括病原体如单核细胞增生李斯特氏菌和某些梭菌属物种以及一些腐败生物，包括嗜热孢子形成细菌和某些酵母。革兰氏阴性细菌由于其复杂的细胞壁结构而对溶菌酶作用更具抗性。

动物实验

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.3449 mL	1.7246 mL	3.4491 mL
5 mM	0.069 mL	0.3449 mL	0.6898 mL
10 mM	0.0345 mL	0.1725 mL	0.3449 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m²)	0.007	0.025	0.15	0.05	0.02	0.5
K_m 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

稳定性

介绍：
分子量：14300。
活力：≥20000u/mg。
适宜pH:5.3～6.4。
来源：鸡蛋清。
性状：粉末。
由18种129个氨基酸残基构成的单一肽链。它富含碱性氨基酸，有4对二硫键维持酶构型，是一种碱性蛋白质，其N端为赖氨酸，C端为亮氨酸。蛋清溶菌酶是C型，是已知的最耐热的酶。
活力定义：一个溶菌酶活力单位定义为 25℃ ,pH6. 2 条件下,使用藤黄微球菌悬浊液在 450nm 处每分钟引起吸光度变化为 0.001 所需溶菌酶的量。

储存条件： 2-8℃

用途：是一种能水解致病菌中黏多糖的碱性酶。主要通过破坏细胞壁中的N-乙酰胞壁酸和N-乙酰氨基葡糖之间的β-1,4糖苷键，使细胞壁不溶性黏多糖分解成可溶性糖肽，导致细胞壁破裂内容物逸出而使细菌溶解。溶菌酶还可与带负电荷的病毒蛋白直接结合，与DNA、RNA、脱辅基蛋白形成复盐，使病毒失活。可分解溶壁微球菌、巨大芽孢杆菌、黄色八叠球菌等革兰阳性菌。

储存条件

储存条件: 2-8°C

运输方式: 冰袋运输，根据产品的不同，可能会有相应调整。

化学品安全说明书(MSDS)

下载MSDS

质检证书(COA)

个人防护装备	Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
危害码 (欧洲)	B
安全声明 (欧洲)	S22;S24/25
危险品运输编码	NONH for all modes of transport
WGK德国	3
RTECS号	OL5989850

1. 物质的识别

产品名：	Lysozyme (from egg white)
CAS号：	12650-88-3
制造商/供应商：	西域试剂网站：www.hzbp.cn 邮件：13911702513@139.com

2. 合成/成分数据

产品名：	Lysozyme (from egg white)
分子式：	C125H196N40O36S2
分子量：	2899.27

3. 急救措施

吸入后：	如果吸入，移至空气新鲜处，如果呼吸困难，给输氧，如呼吸停止，给予人工呼吸。
皮肤接触后：	用大量的水冲洗，移除污染的衣服和鞋子。
眼睛接触后：	检查并取下隐形眼镜，并用大量的水冲洗；呼叫医生。
吞食后：	如果吞食，用大量纯净水漱口；呼叫医生。

4. 消防措施

适当的灭火剂：	雾状水，二氧化碳，干粉或泡沫。
防护设备：	穿戴自给式呼吸器和防护服，以防止与皮肤和眼睛接触。

5. 泄漏应急处理

安全防范措施：	封锁泄漏区域；穿戴自给式呼吸器，防护服和厚橡胶手套。
清洁/收集措施：	使用液体粘合原料（硅藻土，通用粘合剂）吸取精细粉末；使用酒精擦洗表面和设备除去污渍；根据第11条处理被污染的材料。

6. 处理和储存

安全处理说明：	避免吸入和接触皮肤，眼睛及衣物；材料可能略微具有刺激性。
储存：	-20°C, protect from light, dry, sealed

7. 接触控制和个人防护

呼吸设备：	NIOSH / MSHA认可的呼吸器。
双手保护：	耐化学腐蚀的橡胶手套。
眼睛防护：	化学安全护目镜。

8. 稳定性和反应活性

稳定性：	按照说明存储是稳定的；避免强氧化剂。
热分解/其他要避免的情况：	避免光和热。

9. 毒性资料

急性毒性：	无可用资料。
主要刺激性影响：	无可用资料。
在皮肤上：	无可用资料。
对眼睛：	无可用资料；可能具有刺激性。

10. 生态资料

一般注意事项：

无可用资料。

11. 废弃处置

按照所在国家，省份，县市和地方的法规处置。

12. 运输信息

正确的运输名称：	无
非危险品运输：	这种物质被视为非危险品运输。

13. 法规信息

尚未有针对此产品作出的化学安全性评估。

14. 其他信息

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