1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐|N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, ≥98.5%|cas:25952-53-8|西域试剂

1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐,N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, ≥98.5%

产品编号：Sigma-E7750| CAS NO：25952-53-8| MDL NO：MFCD00012503| 分子式：C8H17N3·HCl| 分子量：191.70

EDC.HCl Crosslinker 是一种水溶性碳二亚胺，主要用作多肽、蛋白质、核苷酸合成中的脱水剂，可实现快速多肽缩合反应的水溶性碳二亚胺型缩合剂。碳二亚胺介导耦联的方法是一种最传统的肽键形成方法。为了合成目标化合物，充分混合羧酸和胺后，加入碳二亚胺试剂。反应完成后，碳二亚胺试剂变成相应的尿素。EDC 通过与氨基反应形成可与氨基反应的O-酰基脲中间体。如果该中间体不与氨基反应，则会很快水解并重新释放出羧基基团。在N-羟基硫代琥珀酰亚胺(Sulfo-NHS)存在的情况下，EDC.HCl可以将羧基转变为氨基反应活性的Sulfo-NHS酯。

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	包装	价格	库存与货期	购买量	操作
Sigma-E7750		￥1550.00	杭州北京（现货）
Sigma-E7750		￥5680.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

产品名称

1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐

英文名称

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, ≥98.5%

CAS编号

25952-53-8

产品描述

产品熔点

110-115 °C(lit.)

产品沸点

269.1ºC at 760 mmHg

产品密度

0.877 g/mL at 20 °C(lit.)

产品闪点

107.9ºC

精确质量

191.118927

PSA

27.96000

LogP

1.93390

外观性状

白色至灰白色粉末

蒸气压

0.171mmHg at 25°C

折射率

n20/D 1.461

溶解性

水中溶解度:>20g/100ml 溶于乙醇。

溶解性数据

In Vitro:

H₂O : 100 mg/mL (521.65 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (326.03 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.2165 mL	26.0824 mL	52.1648 mL
5 mM	1.0433 mL	5.2165 mL	10.4330 mL
10 mM	0.5216 mL	2.6082 mL	5.2165 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (13.04 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (13.04 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (13.04 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (13.04 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (13.04 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (13.04 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

体外研究

1-（3-二甲氨基丙基）-3-乙基碳化二亚胺盐酸盐（EDC.HCl）是在水介质中形成酰胺键（肽键）的非常有用的试剂。1-（3-二甲氨基丙基）-3-乙基碳化二亚胺盐酸盐（EDC•HCl）广泛用于制备用于胆固醇生物传感器的聚苯胺碳纳米管，用于HPLC的脂肪族胺柱前衍生，用于DNA研究的分子信标形成，用于钙检测的传感器制备，羧酸的荧光测定和肽的固相微序列测定。1-（3-二甲氨基丙基）-3-乙基碳化二亚胺盐酸盐（EDC•HCl）是一种氨基醛脱氢酶（AMADH）抑制剂，而其对谷氨酸脱羧酶（GAD）的抑制作用不显著。

动物实验

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.2165 mL	26.0824 mL	52.1648 mL
5 mM	1.0433 mL	5.2165 mL	10.433 mL
10 mM	0.5216 mL	2.6082 mL	5.2165 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m²)	0.007	0.025	0.15	0.05	0.02	0.5
K_m 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的K_m系数
	动物 A的K_m系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

稳定性

Stable, but sensitive to moisture. Incompatible with strong acids, strong oxidizing agents, moisture.

储存条件

储存条件: 2-8°C

运输方式: 冰袋运输，根据产品的不同，可能会有相应调整。

化学品安全说明书(MSDS)

下载MSDS

质检证书(COA)

符号	GHS05, GHS07
信号词	Danger
危害声明	H315-H318-H335
警示性声明	P261-P280-P305 + P351 + P338
个人防护装备	dust mask type N95 (US);Eyeshields;Gloves
危害码 (欧洲)	Xi:Irritant
风险声明 (欧洲)	R36/37/38
安全声明 (欧洲)	S26-S36/37/39-S45-S37/39
危险品运输编码	UN 2735 8/PG 3
WGK德国	3
RTECS号	FF2200000
海关编码	29252000

1. 物质的识别

产品名：	EDC.HCl Crosslinker
CAS号：	25952-53-8
制造商/供应商：	西域试剂网站：www.hzbp.cn 邮件：13911702513@139.com

2. 合成/成分数据

产品名：	EDC.HCl Crosslinker
别名：	EDC-HCl
分子式：	C8H18ClN3
分子量：	191.7

3. 急救措施

吸入后：	如果吸入，移至空气新鲜处，如果呼吸困难，给输氧，如呼吸停止，给予人工呼吸。
皮肤接触后：	用大量的水冲洗，移除污染的衣服和鞋子。
眼睛接触后：	检查并取下隐形眼镜，并用大量的水冲洗；呼叫医生。
吞食后：	如果吞食，用大量纯净水漱口；呼叫医生。

4. 消防措施

适当的灭火剂：	雾状水，二氧化碳，干粉或泡沫。
防护设备：	穿戴自给式呼吸器和防护服，以防止与皮肤和眼睛接触。

5. 泄漏应急处理

安全防范措施：	封锁泄漏区域；穿戴自给式呼吸器，防护服和厚橡胶手套。
清洁/收集措施：	使用液体粘合原料（硅藻土，通用粘合剂）吸取精细粉末；使用酒精擦洗表面和设备除去污渍；根据第11条处理被污染的材料。

6. 处理和储存

安全处理说明：	避免吸入和接触皮肤，眼睛及衣物；材料可能略微具有刺激性。
储存：	-20°C, dry, sealed

7. 接触控制和个人防护

呼吸设备：	NIOSH / MSHA认可的呼吸器。
双手保护：	耐化学腐蚀的橡胶手套。
眼睛防护：	化学安全护目镜。

8. 稳定性和反应活性

稳定性：	按照说明存储是稳定的；避免强氧化剂。
热分解/其他要避免的情况：	避免光和热。

9. 毒性资料

急性毒性：	无可用资料。
主要刺激性影响：	无可用资料。
在皮肤上：	无可用资料。
对眼睛：	无可用资料；可能具有刺激性。

10. 生态资料

一般注意事项：

无可用资料。

11. 废弃处置

按照所在国家，省份，县市和地方的法规处置。

12. 运输信息

正确的运输名称：	无
非危险品运输：	这种物质被视为非危险品运输。

13. 法规信息

尚未有针对此产品作出的化学安全性评估。

14. 其他信息

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