Benzyl-α-GalNAc|cas:3554-93-6|西域试剂

Benzyl-α-GalNAc,99.89%

产品编号：Bellancom-129389| CAS NO：3554-93-6| 分子式：C₁₅H₂₁NO₆| 分子量：311.33

O-glycosylation-IN-1 是一种有效的 O-糖基化 (O-glycosylation) 抑制剂，用于减少细胞表面的粘蛋白。O-glycosylation-IN-1 抑制人胰腺癌细胞模型黏蛋白 O-糖基化的合成。

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-129389		￥2000.00	杭州北京（现货）
Bellancom-129389		￥5500.00	杭州北京（现货）

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Benzyl-α-GalNAc

产品介绍

Benzyl-α-GalNAc 是一种有效的 O-糖基化 (O-glycosylation) 抑制剂。Benzyl-α-GalNAc 能有效抑制 LX-2 细胞的增殖和活化，并抑制胶原蛋白 I/III 的表达，在肝纤维化中具有较好的研究潜力。Benzyl-α-GalNAc 还可通过抑制 O-糖基化显著增强 5-FU (HY-90006) 的抗肿瘤 (如胰腺癌) 活性。

生物活性

Benzyl-α-GalNAc is a potent O-glycosylation inhibitor. Benzyl-α-GalNAc effectively inhibits the proliferation and activation of LX-2 cells and suppresses the expression of collagen I/III, which has good potential for investigation in liver fibrosis. Benzyl-α-GalNAc also significantly enhances the anti-tumour activity of 5-FU (HY-90006) (e.g. pancreatic cancer) by inhibiting O-glycosylation.

体外研究

Benzyl-α-GalNAc (5 mM; 72 h) inhibits O-glycosylation of mucin in SUIT-2 cells.
Benzyl-α-GalNAc (2, 4 mM; 48 h) inhibits the proliferation and activation of LX-2 cells.
Benzyl-α-GalNAc (2, 4 mM; 48 h) decreases collagen expression in LX-2 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	SUIT-2 cells
Concentration:	5 mM
Incubation Time:	72 h
Result:	Inhibited mucin O-glycosylation.

Cell Viability Assay

Cell Line:	LX-2 cells
Concentration:	2, 4 mM
Incubation Time:	48 h
Result:	Remarkably decreased the expression of α-SMA in a dose-dependent manner (α-SMA is the marker for the activation of HSCs, which means that quiescent cells transform into myofibroblasts).

Cell Proliferation Assay

Cell Line:	LX-2 cells (PDGF-BB-induced)
Concentration:	2, 4 mM
Incubation Time:	48 h
Result:	Partly reversed PDGF-BB-induced proliferation in cells.

Western Blot Analysis

Cell Line:	LX-2 cells
Concentration:	2, 4 mM
Incubation Time:	48 h
Result:	Downregulated the mRNA levels of collagens I and III in a dose-dependent manner.

体内研究
(In Vivo)

Benzyl-α-GalNAc (1 mg/mice; tumoural injection; single daily; days 4, 6, 8 and 10 after tumour size reaches 50-70 mm³) enhances the anti-tumour activity of 5-FU by inhibiting O-glycosylation in mice.
(Mucin overexpression limits the effectiveness of 5-FU by reducing intracellular drug uptake and antineoplastic drug effects in pancreatic tumours)

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female severe combined immunodeficient (SCID) mice (6 to 8-week-old; capan-1 tumour model).
Dosage:	1 mg/mice (in combination with 5-FU)
Administration:	Tumoural injection; single daily; days 4, 6, 8 and 10 after tumour size reaches 50-70 mm³
Result:	Significantly lowered neoplastic cells compared to 5-FU alone.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female severe combined immunodeficient (SCID) mice (6 to 8-week-old; capan-1 tumour model).
Dosage:	1 mg/mice (in combination with 5-FU)
Administration:	Tumoural injection; single daily; days 4, 6, 8 and 10 after tumour size reaches 50-70 mm³
Result:	Significantly lowered neoplastic cells compared to 5-FU alone.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female severe combined immunodeficient (SCID) mice (6 to 8-week-old; capan-1 tumour model).
Dosage:	1 mg/mice (in combination with 5-FU)
Administration:	Tumoural injection; single daily; days 4, 6, 8 and 10 after tumour size reaches 50-70 mm³
Result:	Significantly lowered neoplastic cells compared to 5-FU alone.

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (80.30 mM; ultrasonic and warming and heat to 60°C)

H₂O : 5 mg/mL (16.06 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2120 mL	16.0601 mL	32.1203 mL
5 mM	0.6424 mL	3.2120 mL	6.4241 mL
10 mM	0.3212 mL	1.6060 mL	3.2120 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 3.33 mg/mL (10.70 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.03 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (8.03 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.03 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

参考文献

. Kalra AV, et al. Mucin impedes cytotoxic effect of 5-FU against growth of human pancreatic cancer cells: overcoming cellular barriers for therapeutic gain. Br J Cancer. 2007 Oct 8;97(7):910-8. Epub 2007 Oct 2. [Content Brief]

. Kalra AV, et al. Mucin overexpression limits the effectiveness of 5-FU by reducing intracellular drug uptake and antineoplastic drug effects in pancreatic tumours. Eur J Cancer. 2009 Jan;45(1):164-73. [Content Brief]

. Fan X, et al. Protein O glycosylation regulates activation of hepatic stellate cells. Inflammation. 2013 Dec;36(6):1248-52. https://pubmed.ncbi.nlm.nih.gov/23743764/ [Content Brief]

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产品编号(Item Number)

批号(Lot Number)

个人防护装备	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
危害码 (欧洲)	Xi: Irritant;
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WGK德国	3

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Benzyl-α-GalNAc,99.89%

Benzyl-α-GalNAc

化学品安全说明书(MSDS)

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