Tubulin inhibitor 11|cas:2366260-33-3|西域试剂

Tubulin inhibitor 11,99.92%

产品编号：Bellancom-152156| CAS NO：2366260-33-3| 分子式：C₂₂H₂₃N₃O₃S| 分子量：409.50

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货号	价格	库存与货期
Bellancom-152156	￥1500.00	杭州北京（现货）
Bellancom-152156	￥2500.00	杭州北京（现货）
Bellancom-152156	￥5500.00	杭州北京（现货）
Bellancom-152156	￥8500.00	杭州北京（现货）
Bellancom-152156	￥13500.00	杭州北京（现货）

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Tubulin inhibitor 11

产品介绍

Tubulin inhibitor 11 是一种有效的具有口服活性的微管蛋白 (tubulin) 抑制剂。Tubulin inhibitor 11 靶向微管蛋白上的秋水仙碱结合位点，抑制微管蛋白聚合，促进有丝分裂阻断和细胞凋亡 (apoptosis)。

生物活性

Tubulin inhibitor 11 is a potent and orally active tubulin inhibitor. Tubulin inhibitor 11 targets the Colchicine binding site on tubulin, inhibits tubulin polymerization, promotes mitotic blockade and apoptosis.

体外研究

Tubulin inhibitor 11 (Compound 48; 0.03-0.1 μM; 12 hours) causes cell cycle arrest at G2/M phase. Tubulin inhibitor 11 reduces cdc2 phosphorylation and increases cyclin B1 and phosphorylation of histone H3 at Ser10 in cells.
Tubulin inhibitor 11 (Compound 48; 0.03-0.3 μM; 48 hours) causes cancer cell apoptosis.
Tubulin inhibitor 11 (Compound 48) demonstrates favorable antiproliferative activities in DU145 cells with an IC₅₀ of 8 nM.
Tubulin inhibitor 11 (Compound 48; 0.03-0.3 μM) significantly reduces the expression levels of acetyl-α-tubulin and α-tubulin in a dose-dependent manner.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis

Cell Line:	DU145 cells
Concentration:	0.01 μM, 0.03 μM, and 0.1 μM
Incubation Time:	12 hours
Result:	Caused cell cycle arrest at G2/M phase.

Apoptosis Analysis

Cell Line:	DU145 cells
Concentration:	0.03 μM, 0.1 μM, and 0.3 μM
Incubation Time:	48 hours
Result:	Induced cancer cell apoptosis.

Western Blot Analysis

Cell Line:	DU145 cells
Concentration:	0.01 μM, 0.03 μM, and 0.1 μM
Incubation Time:	12 hours
Result:	Reduced cdc2 phosphorylation and increased cyclin B1 and phosphorylation of histone H3 at Ser10 in cells.

体内研究
(In Vivo)

Tubulin inhibitor 11 (Compound 48; 5-10 mg/kg; p.o; once daily) shows robust in vivo antitumor efficacies.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic BALB/c nude mice (18-20 g) injected with DU145, NCI-H1299 or A549R cells
Dosage:	5 mg/kg, 10 mg/kg
Administration:	p.o; once daily
Result:	Effectively suppressed tumor growth.

体内研究

Tubulin inhibitor 11 (Compound 48; 5-10 mg/kg; p.o; once daily) shows robust in vivo antitumor efficacies.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic BALB/c nude mice (18-20 g) injected with DU145, NCI-H1299 or A549R cells
Dosage:	5 mg/kg, 10 mg/kg
Administration:	p.o; once daily
Result:	Effectively suppressed tumor growth.

体内研究

Tubulin inhibitor 11 (Compound 48; 5-10 mg/kg; p.o; once daily) shows robust in vivo antitumor efficacies.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic BALB/c nude mice (18-20 g) injected with DU145, NCI-H1299 or A549R cells
Dosage:	5 mg/kg, 10 mg/kg
Administration:	p.o; once daily
Result:	Effectively suppressed tumor growth.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (244.20 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4420 mL	12.2100 mL	24.4200 mL
5 mM	0.4884 mL	2.4420 mL	4.8840 mL
10 mM	0.2442 mL	1.2210 mL	2.4420 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.11 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.11 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.11 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。