CB-6644|cas:2316817-88-4|西域试剂

CB-6644,99.88%

产品编号：Bellancom-114429| CAS NO：2316817-88-4| 分子式：C₂₉H₃₄ClFN₄O₅| 分子量：573.06

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货号	价格	库存与货期
Bellancom-114429	￥3500.00	杭州北京（现货）
Bellancom-114429	￥9800.00	杭州北京（现货）
Bellancom-114429	￥11700.00	杭州北京（现货）
Bellancom-114429	￥23000.00	杭州北京（现货）
Bellancom-114429	￥34000.00	杭州北京（现货）

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CB-6644

产品介绍

CB-6644 一种选择性的 RUVBL1/2 复合体抑制剂。抑制 RUVBL1/2 的 ATP 酶活性，IC₅₀ 为 15 nM。具有抗癌活性。

生物活性

CB-6644 is a selective inhibitor of RUVBL1/2 complex with anti-cancer activity. CB-6644 blocks the ATPase activity of RUVBL1/2 with an IC₅₀ of 15 nM.

体外研究

CB-6644 (20 µM) interacts with the RUVBL1/2 complex in Ramos cells.
CB-6644 (0.001-10 μM; 72 hours) potently kills 123 cell lines (including HCT116, NCI-1975, and HT29 cells) with an EC₅₀ range of 41 to 785 nM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	123 cell lines such as HCT116, NCI-1975, and HT29 cells
Concentration:	0.001, 0.01, 0.1, 1, and 10 μM
Incubation Time:	72 hours
Result:	Potently killed cells with an EC₅₀ range of 41 to 785 nM.

体内研究
(In Vivo)

CB-6644 (150 mg/kg, oral administration, 10 days of treatment for SCID-beige mice bearing Ramos xenograft models, 30 days of treatment for SCID-beige mice bearing RPMI8226 xenograft models) has antitumor activity in xenograft tumor models with tumor growth inhibitions (TGIs) of 68 and 81%, respectively in Ramos xenograft models and RPMI8226 xenograft models.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID-beige mice bearing human tumor xenografts derived from either Burkitt’s lymphoma (Ramos) or multiple myeloma (RPMI8226) cell lines
Dosage:	150 mg/kg
Administration:	Oral gavage once (qd) or twice (BID) daily, 10 days of treatment for Ramos, 30 days of treatment for RPMI8226
Result:	There was no significant bodyweight loss in mice. TGI of 68 and 81% in Ramos and RPMI8226, respectively.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID-beige mice bearing human tumor xenografts derived from either Burkitt’s lymphoma (Ramos) or multiple myeloma (RPMI8226) cell lines
Dosage:	150 mg/kg
Administration:	Oral gavage once (qd) or twice (BID) daily, 10 days of treatment for Ramos, 30 days of treatment for RPMI8226
Result:	There was no significant bodyweight loss in mice. TGI of 68 and 81% in Ramos and RPMI8226, respectively.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID-beige mice bearing human tumor xenografts derived from either Burkitt’s lymphoma (Ramos) or multiple myeloma (RPMI8226) cell lines
Dosage:	150 mg/kg
Administration:	Oral gavage once (qd) or twice (BID) daily, 10 days of treatment for Ramos, 30 days of treatment for RPMI8226
Result:	There was no significant bodyweight loss in mice. TGI of 68 and 81% in Ramos and RPMI8226, respectively.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (174.50 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7450 mL	8.7251 mL	17.4502 mL
5 mM	0.3490 mL	1.7450 mL	3.4900 mL
10 mM	0.1745 mL	0.8725 mL	1.7450 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.63 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.63 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (3.63 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.63 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。