CASIN|cas:425399-05-9|西域试剂

CASIN,99.82%

产品编号：Bellancom-12874| CAS NO：425399-05-9| 分子式：C₂₀H₂₂N₂O| 分子量：306.40

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货号	价格	库存与货期
Bellancom-12874	￥770.00	杭州北京（现货）
Bellancom-12874	￥1300.00	杭州北京（现货）
Bellancom-12874	￥2800.00	杭州北京（现货）
Bellancom-12874	￥4900.00	杭州北京（现货）
Bellancom-12874	￥7900.00	杭州北京（现货）

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CASIN

产品介绍

CASIN 是一个选择性的 GTPase Cdc42 抑制剂，其 IC₅₀ 值是 2 uM。CASIN 可用于癌症的研究。

生物活性

CASIN is a selective GTPase Cdc42 inhibitor with an IC₅₀ of 2 uM. CASIN can be used for the research of cancer.

体外研究

CASIN (0-10 μM; 3 d) inhibits the growth of AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells.
CASIN (0-10 μM; 16 h) inhibits melphalan induced FANCD2 mono-ubiquitination in LR5 cells but shows no effect to S cells.
CASIN (5 μM; 2 d) increases cell apoptosis of bortezomib-resistant MM patient cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells
Concentration:	0-10 μM
Incubation Time:	3 days
Result:	Inhibited MM cell lines including AMO-1, ARH-77, IM-9 , JJN-3, L-363, MOLP-8 and RPMI-8226/S cells with GI₅₀s vof 3.23, 4.31, 4.87, 2.56, 4.64, 4.67 and 3.97 μM, respectively.

Western Blot Analysis

Cell Line:	MM (S) and melphalan-resistant (LR5) MM cells
Concentration:	5 μM
Incubation Time:	16 hours
Result:	Abolished melphalan induced FANCD2 mono-ubiquitination and DNA damage in LR5, sensitized LR5 to melphalan-induced cell death by blocking FANCD2-mediated DNA damage repair.

Apoptosis Analysis

Cell Line:	Bortezomib-resistant MM patients cells
Concentration:	5 μM
Incubation Time:	2 days
Result:	2-fold increased cell apoptosis in the cell derived from bortezomib-resistant MM patients.

体内研究
(In Vivo)

CASIN (20 mg/kg; i.p. 2 times a day) prolongs lifetime of mice with busulfan injection.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NSG mice with busulfan injection
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; 20 mg/kg 2 times a day
Result:	Significantly prolonged the lifespan of xenografts mice better than bortezomib, and showed a leading position when combined with bortezomib.

体内研究

CASIN (20 mg/kg; i.p. 2 times a day) prolongs lifetime of mice with busulfan injection.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NSG mice with busulfan injection
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; 20 mg/kg 2 times a day
Result:	Significantly prolonged the lifespan of xenografts mice better than bortezomib, and showed a leading position when combined with bortezomib.

体内研究

CASIN (20 mg/kg; i.p. 2 times a day) prolongs lifetime of mice with busulfan injection.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NSG mice with busulfan injection
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; 20 mg/kg 2 times a day
Result:	Significantly prolonged the lifespan of xenografts mice better than bortezomib, and showed a leading position when combined with bortezomib.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 31 mg/mL (101.17 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2637 mL	16.3185 mL	32.6371 mL
5 mM	0.6527 mL	3.2637 mL	6.5274 mL
10 mM	0.3264 mL	1.6319 mL	3.2637 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.79 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.79 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.79 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。