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    • Phosphoramide mustard (cyclohexanamine),95.0%

    产品编号：Bellancom-137316A| CAS NO：1566-15-0| 分子式：C10H24Cl2N3O2P| 分子量：320.20

    本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

    货号 包装 价格 库存与货期 购买量 操作
    Bellancom-137316A
    		￥3400.00 杭州 北京（现货）
    Bellancom-137316A
    		￥6000.00 杭州 北京（现货）
    Bellancom-137316A
    		￥12500.00 杭州 北京（现货）

    增值税发票√顺丰快递√订货电话：18601927057

    Phosphoramide mustard (cyclohexanamine)

    产品介绍 Phosphoramide mustard cyclohexanamine 是环磷酰胺 (HY-17420) 的活性代谢物，具有抗肿瘤活性。Phosphoramide mustard cyclohexanamine 能诱导 DNA 损伤。
    生物活性

    Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage.

    体外研究

    Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.
    Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs).
    Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.
    Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.

    西域 has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay

    Cell Line: SIGCs
    Concentration: 0.5 μM, 1 μM, 3 μM, 6 μM
    Incubation Time: 48 hours
    Result: Reduced cell viability at concentrations of 3 μM and higher.

    RT-PCR

    Cell Line: SIGCs
    Concentration: 3 μM, 6 μM
    Incubation Time: 24 hours, 48 hours
    Result: Increased DDR gene mRNA expression levels.

    Western Blot Analysis

    Cell Line: SIGCs
    Concentration: 3 μM, 6 μM
    Incubation Time: 24 hours, 48 hours
    Result: Generally increased DDR proteins.
    体内研究
    (In Vivo)

    Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats.
    Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg).

    西域 has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor
    Dosage: 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
    Administration: Intraperitoneal injection, once daily, for 5 consecutive days
    Result: Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
    Animal Model: Rats
    Dosage: 86.0 mg/kg (Pharmacokinetic Analysis)
    Administration: Intravenous injection
    Result: T1/2 (15.1 min).
    体内研究

    Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats.
    Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg).

    西域 has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor
    Dosage: 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
    Administration: Intraperitoneal injection, once daily, for 5 consecutive days
    Result: Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
    Animal Model: Rats
    Dosage: 86.0 mg/kg (Pharmacokinetic Analysis)
    Administration: Intravenous injection
    Result: T1/2 (15.1 min).
    体内研究

    Phosphoramide mustard cyclohexanamine (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats.
    Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg).

    西域 has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor
    Dosage: 2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
    Administration: Intraperitoneal injection, once daily, for 5 consecutive days
    Result: Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
    Animal Model: Rats
    Dosage: 86.0 mg/kg (Pharmacokinetic Analysis)
    Administration: Intravenous injection
    Result: T1/2 (15.1 min).
    性状Solid
    溶解性数据
    In Vitro: 

    H2O : 100 mg/mL (312.30 mM; Need ultrasonic)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.1230 mL 15.6152 mL 31.2305 mL
    5 mM 0.6246 mL 3.1230 mL 6.2461 mL
    10 mM 0.3123 mL 1.5615 mL 3.1230 mL
    *

    请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

    In Vivo:

    请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
    分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 1.

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL (312.30 mM); Clear solution; Need ultrasonic

    *以上所有助溶剂都可在 西域 网站选购。
    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    -20°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

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