STF-31|cas:724741-75-7|西域试剂

STF-31,98.01%

产品编号：Bellancom-18728| CAS NO：724741-75-7| 分子式：C₂₃H₂₅N₃O₃S| 分子量：423.53

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-18728		￥1100.00	杭州北京（现货）
Bellancom-18728		￥4400.00	杭州北京（现货）

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STF-31

产品介绍

STF-31 是一种葡萄糖转运蛋白 1 (GLUT1) 的选择性抑制剂，IC₅₀ 值为 1 μM。STF-31 也是一种 NAMPT 抑制剂。STF-31 抑制肾细胞癌 (RCC) 4 细胞中的葡萄糖摄取。

生物活性

STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC₅₀ of 1 μM. STF-31 is also a NAMPT inhibitor. STF-31 inhibits glucose uptake in renal cell carcinoma (RCC) 4 cells.

体外研究

STF-31 (0.01-10 μM; 10 days) is specifically toxic to RCC4 cells, whereas RCC4/VHL cells are relatively unaffected. RCC4/VHL cells treated with STF-31 (5 μM; 10 days) largely recovery, whereas RCC4 cells under the same conditions does not.
STF-31 (1.25-5 μM; 3 days) does not induce autophagy, apoptosis, or DNA damage. STF-31 causes a necrotic cell death.
STF-31 reduces the efferocytosis of wild type and SLC2A1-overexpressing LR73 cells^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Wild-type RCC4 cells (RCC4/VHL) and RCC4 cells without VHL
Concentration:	0.01, 0.1, 1, 10 μM
Incubation Time:	10 days
Result:	Reduced viability of RCC4 cells without VHL in a concentration-dependent manner when compared to their wild-type counterparts.

体内研究
(In Vivo)

STF-31 (10 mg/kg; i.p.; twice daily for 2 days, followed by once daily for another 3 days) does not affect normal mice body weight, behavior, and ERG responses. STF-31 reduces light-induced CX3CR1^gfp/+ mice microglial activation and retinal degeneration.
STF-31 (10 mg/kg; i.p.) promotes accumulation of necrotic thymocytes after Dexamethasone-induced apoptosis in vivo^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Twelve-week old C57BL/6J and CX3CR1^gfp/+ mice
Dosage:	10 mg/kg
Administration:	I.p. injections; twice daily for 2 days, followed by once daily for another 3 days
Result:	Improved photoreceptor survival and reduced microglial activation of CX3CR1^gfp/+ mice, and not induced any C57BL/6J mice retinal cell death.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Twelve-week old C57BL/6J and CX3CR1^gfp/+ mice
Dosage:	10 mg/kg
Administration:	I.p. injections; twice daily for 2 days, followed by once daily for another 3 days
Result:	Improved photoreceptor survival and reduced microglial activation of CX3CR1^gfp/+ mice, and not induced any C57BL/6J mice retinal cell death.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Twelve-week old C57BL/6J and CX3CR1^gfp/+ mice
Dosage:	10 mg/kg
Administration:	I.p. injections; twice daily for 2 days, followed by once daily for another 3 days
Result:	Improved photoreceptor survival and reduced microglial activation of CX3CR1^gfp/+ mice, and not induced any C57BL/6J mice retinal cell death.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 34 mg/mL (80.28 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.3611 mL	11.8055 mL	23.6111 mL
5 mM	0.4722 mL	2.3611 mL	4.7222 mL
10 mM	0.2361 mL	1.1806 mL	2.3611 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.5 mg/mL (5.90 mM); Clear solution; Need ultrasonic

此方案可获得 2.5 mg/mL (5.90 mM) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (5.90 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.90 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明