IRG1-IN-1|cas:2407652-42-8|西域试剂

IRG1-IN-1,99.82%

产品编号：Bellancom-148335| CAS NO：2407652-42-8| 分子式：C₁₈H₁₅FO₄| 分子量：314.31

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-148335	￥1500.00	杭州北京（现货）
Bellancom-148335	￥2500.00	杭州北京（现货）
Bellancom-148335	￥5500.00	杭州北京（现货）
Bellancom-148335	￥9500.00	杭州北京（现货）
Bellancom-148335	￥15000.00	杭州北京（现货）

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IRG1-IN-1

产品介绍

IRG1-IN-1 是衣康酸衍生物。IRG1-IN-1 可以抑制免疫反应基因 1 (IRG1) 的活性。IRG1-IN-1 可用于癌症、炎症和自身免疫性疾病的研究。

生物活性

IRG1-IN-1 is an itaconic acid derivative. IRG1-IN-1 can inhibit immune-responsive gene 1 (IRG1) activity. IRG1-IN-1 can be used for the research of cancer, inflammation and autoimmune diseases.

体外研究

IRG1-IN-1(compound 6) (0.5 mM; 2 mM) reduces production of itaconic acid and the secretion of TNFα from LPS-stimulated human monocyte derived macrophages (hMDMs) .
IRG1-IN-1 (0.5 mM; 1 mM) inhibits the proliferation of C-IRG1-9 rat glioma cells.
IRG1-IN-1(10 nM) increases proliferation of TCR-activated hCD8⁺ T cells.
IRG1-IN-1(10μM) shows depletion of trimethylation of histone 3 at lysine 4 (H3K4me3) in CR-activated hCD8⁺ T cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	TCR-activated hCD8⁺ T cells
Concentration:	10 μM
Incubation Time:	24-72 h
Result:	Decreased protein levels of histone 3 (H3).

Cell Proliferation Assay

Cell Line:	C-IRG1-9 rat glioma cells and TCR-activated hCD8⁺ T cells
Concentration:	0.5 mM; 1 mM; 10 nM
Incubation Time:	48-96 h
Result:	Inhibited the proliferation of C-IRG1-9 rat glioma cells and increased proliferation of TCR-activated hCD8⁺ T cells.

体内研究
(In Vivo)

IRG1-IN-1 (compound 6) (i.p.; 0.2 mg/kg; 27 days) shows antitumor effect in C57BL/6 mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice
Dosage:	0.2 mg/kg
Administration:	IP; 27 days
Result:	Increased survival of C57BL/6 mice bearing mouse CT26 colorectal tumors. Decreased intratumoral frequency of M-MDSCs in tumors.

体内研究

IRG1-IN-1 (compound 6) (i.p.; 0.2 mg/kg; 27 days) shows antitumor effect in C57BL/6 mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice
Dosage:	0.2 mg/kg
Administration:	IP; 27 days
Result:	Increased survival of C57BL/6 mice bearing mouse CT26 colorectal tumors. Decreased intratumoral frequency of M-MDSCs in tumors.

体内研究

IRG1-IN-1 (compound 6) (i.p.; 0.2 mg/kg; 27 days) shows antitumor effect in C57BL/6 mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice
Dosage:	0.2 mg/kg
Administration:	IP; 27 days
Result:	Increased survival of C57BL/6 mice bearing mouse CT26 colorectal tumors. Decreased intratumoral frequency of M-MDSCs in tumors.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (318.16 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.1816 mL	15.9079 mL	31.8157 mL
5 mM	0.6363 mL	3.1816 mL	6.3631 mL
10 mM	0.3182 mL	1.5908 mL	3.1816 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.95 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.95 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.95 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。