MPP dihydrochloride|cas:911295-24-4|西域试剂

MPP dihydrochloride,99.03%

产品编号：Bellancom-103454| CAS NO：911295-24-4| 分子式：C₂₉H₃₃Cl₂N₃O₃| 分子量：542.50

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货号	价格	库存与货期
Bellancom-103454	￥3500.00	杭州北京（现货）
Bellancom-103454	￥5550.00	杭州北京（现货）
Bellancom-103454	￥10500.00	杭州北京（现货）

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MPP dihydrochloride

产品介绍

MPP dihydrochloride 是一种强效的选择性 ER (雌激素受体)调节剂。MPP dihydrochloride 可诱导子宫内膜癌和 oLE 细胞凋亡。MPP dihydrochloride 可逆转 β - 雌激素的积极作用。在体内，MPP dihydrochloride 对小鼠子宫 ERalpha 具有激动剂和拮抗剂的混合作用。

生物活性

MPP dihydrochloride is a potent and selective ER (estrogen receptor) modulator. MPP dihydrochloride induces significant apoptosis in the endometrial cancer and oLE cell lines. MPP dihydrochloride reverses the positive effects of beta-estradiol. MPP dihydrochloride has mixed agonist/antagonist action on murine uterine ERalpha in vivo.

体外研究

MPP (1, 5, 10, 25, 50 and 100 µM; 24 h) decreases cell viability with an IC₅₀ value of 20.01 µM in RL95-2 cells.
MPP dihydrochloride shows antiproliferative activity at a concentration of 10 μM in RL95-2 cells.
MPP dihydrochloride (20 µM; 24 h) reduces the phosphorylation of ERα, while it does not alter the phosphorylation of Akt. MPP dihydrochloride reduces the ratio of p-ERα/ERα.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	RL95-2 endometrium cancer cells
Concentration:	1, 5, 10, 25, 50 and 100 µM
Incubation Time:	24 hours
Result:	The treatment with 25 µM, 50 µM and 100 µM for 24 h decreased cell viability significantly. However, cell viability was not significantly changed by MPP dihydrochloride at concentration below 25 µM.

Cell Proliferation Assay

Cell Line:	RL95-2 cell
Concentration:	10, 15, 20 and 25 µM
Incubation Time:	72 hours
Result:	Showed antiproliferative activity at a concentration of 10 μM.

Western Blot Analysis

Cell Line:	RL95-2 cell line
Concentration:	20 µM
Incubation Time:	24 hours
Result:	Reduced the phosphorylation of ERα, while it did not alter the phosphorylation of Akt. Reduced the ratio of p-ERα/ERα compared to the control group.

体内研究
(In Vivo)

MPP (Low dose 20 μg/kg body weight or high dose 200 μg/kg body weight) leads to a dose-dependent attenuation of percent prepulse inhibition (PPI).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6N mice at the age of 9-10 weeks
Dosage:	Low dose (20 μg/kg body weight) or high dose (200 μg/kg body weight)
Administration:	Administered subcutaneously (s.c.) injected; injection volume of 5 mL/kg; 60 min before PPI testing
Result:	Led to a dose-dependent attenuation of percent PPI. Pretreatment with 200 μg/kg reduced the mean percent PPI scores by ~30%.

体内研究

MPP (Low dose 20 μg/kg body weight or high dose 200 μg/kg body weight) leads to a dose-dependent attenuation of percent prepulse inhibition (PPI).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6N mice at the age of 9-10 weeks
Dosage:	Low dose (20 μg/kg body weight) or high dose (200 μg/kg body weight)
Administration:	Administered subcutaneously (s.c.) injected; injection volume of 5 mL/kg; 60 min before PPI testing
Result:	Led to a dose-dependent attenuation of percent PPI. Pretreatment with 200 μg/kg reduced the mean percent PPI scores by ~30%.

体内研究

MPP (Low dose 20 μg/kg body weight or high dose 200 μg/kg body weight) leads to a dose-dependent attenuation of percent prepulse inhibition (PPI).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6N mice at the age of 9-10 weeks
Dosage:	Low dose (20 μg/kg body weight) or high dose (200 μg/kg body weight)
Administration:	Administered subcutaneously (s.c.) injected; injection volume of 5 mL/kg; 60 min before PPI testing
Result:	Led to a dose-dependent attenuation of percent PPI. Pretreatment with 200 μg/kg reduced the mean percent PPI scores by ~30%.

性状

Solid

溶解性数据

In Vitro:

DMSO : 30 mg/mL (55.30 mM; Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8433 mL	9.2166 mL	18.4332 mL
5 mM	0.3687 mL	1.8433 mL	3.6866 mL
10 mM	0.1843 mL	0.9217 mL	1.8433 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

参考文献