Pulsatilla saponin D 白头翁皂苷D; SB365; Hederacolchiside A|cas:68027-15-6|西域试剂

Pulsatilla saponin D 白头翁皂苷D; SB365; Hederacolchiside A,98.60%

产品编号：Bellancom-N0834| CAS NO：68027-15-6| 分子式：C₄₇H₇₆O₁₇| 分子量：913.10

Pulsatilla saponin D(SB365)可从Pulsatilla koreana的根中提取，可作为化疗保护剂。

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N0834		￥1700.00	杭州北京（现货）

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Pulsatilla saponin D 白头翁皂苷D; SB365; Hederacolchiside A

产品介绍

Pulsatilla saponin D (SB365) 可从 Pulsatilla chinensis根部分离得到，具有抗癌活性。

生物活性

Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla chinensis, is an anti-tumor agent^[4].

体外研究

Pulsatilla saponin D (SB365) shows apoptotic effect accompanied by increased evidence of cleaved caspase-3 and poly (ADP ribose) polymerase.
Pulsatilla saponin D (SB365) strongly inhibits c-Met expression in gastric cancer cells.
Pulsatilla saponin D (SB365) strongly suppresses the growth and proliferation of 5 human pancreatic cancer cell lines (MIAPaCa-2, BXPC-3, PANC-1, AsPC-1 and HPAC) .
Pulsatilla saponin D (SB365) shows anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF.
Pulsatilla saponin D (0.3 ng/mL to 10 μg/mL) exhibits anti-tumor activity in NSCLC cancer cells^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:	A-549, SKMEL-2, MCF-7, and Lewis lung carcinoma (LLC) cells.
Concentration:	0.3 ng/mL to 10 μg/mL.
Incubation Time:	72 h.
Result:	Exhibited an ED₅₀ value of 6.3 μg/mL for A-549 cells.

体内研究
(In Vivo)

Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	2x2x2 mm³ tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice^[4].
Dosage:	6.4 mg/kg.
Administration:	Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result:	Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).

体内研究

Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	2x2x2 mm³ tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice^[4].
Dosage:	6.4 mg/kg.
Administration:	Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result:	Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).

体内研究

Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	2x2x2 mm³ tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice^[4].
Dosage:	6.4 mg/kg.
Administration:	Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result:	Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 39 mg/mL (42.71 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.0952 mL	5.4759 mL	10.9517 mL
5 mM	0.2190 mL	1.0952 mL	2.1903 mL
10 mM	0.1095 mL	0.5476 mL	1.0952 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (2.28 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (2.28 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (2.28 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

参考文献

. Hong SW, et al. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis. 2013 Sep;34(9):2156-69. [Content Brief]

. Son MK, et al. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep. 2013 Aug;30(2):801-8. [Content Brief]

. Son MK, et al. SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway. Food Chem. 2013 Jan 1;136(1):26-33. [Content Brief]

[4]. Yong Kim, et al. Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res. 2004 Sep;27(9):915-8. [Content Brief]

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Pulsatilla saponin D 白头翁皂苷D; SB365; Hederacolchiside A,98.60%

Pulsatilla saponin D 白头翁皂苷D; SB365; Hederacolchiside A

化学品安全说明书(MSDS)

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