ZZW-115 hydrochloride|cas:10122-45-9|西域试剂

ZZW-115 hydrochloride,98.00%

产品编号：Bellancom-111838A| CAS NO：10122-45-9| 分子式：C₂₄H₃₄Cl₃F₃N₄S| 分子量：573.97

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-111838A	￥2500.00	杭州北京（现货）
Bellancom-111838A	￥3800.00	杭州北京（现货）
Bellancom-111838A	￥8000.00	杭州北京（现货）
Bellancom-111838A	￥12800.00	杭州北京（现货）

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ZZW-115 hydrochloride

产品介绍

ZZW-115 hydrochloride 是一种有效的 NUPR1 抑制剂，K_d 为 2.1 μM，通过坏死和凋亡 (apoptosis) 诱导肿瘤细胞死亡。具有抗癌活性。

生物活性

ZZW-115 hydrochloride is a potent NUPR1 inhibitor, with a K_d of 2.1 μM. ZZW-115 hydrochloride induces tumor cell death by necroptosis and apoptosis. Anticancer activity.

体外研究

ZZW-115 hydrochloride (0.1-33 μM; 72 hours) is efficient in killing cancer cells, with an IC₅₀ in the range of 0.84 μM (ANOR) to 4.93 μM (HN14).
ZZW-115 hydrochloride (0-100 μM; 24-72 hours) is efficient to kill these tumor cells with an IC₅₀ in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells).
ZZW-115 hydrochloride induces pancreatic cell death by necrosis and apoptosis. ZZW-115 hydrochloride treatment induces a decrease in ATP production and induces a ROS overproduction.
LDH release is significantly higher in ZZW-115 hydrochloride-treated cells (MiaPaCa-2, 02-063, LIPC, Foie8b, and HN14 cells) than in control cells in a concentration-dependent manner. Similarly, caspase 3/7 activity is also greater in ZZW-115 hydrochloride-treated cells. These experiments demonstrated that ZZW-115 hydrochloride exerted both pronecrotic and proapoptotic effects.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	ANOR cells, MiaPaCa-2, 02-063, 01008, LIPC, 02136, HN01,01046, AOIPC, Foie8b, HN14 cells
Concentration:	0.1- 33 μM
Incubation Time:	72 hours
Result:	Was efficient in killing cancer cells, with an IC₅₀ in the range of 0.84 μM (ANOR) to 4.93 μM (HN14).

Cell Proliferation Assay

Cell Line:	U87, A375, U2OS, SaOS-2, HT29, SK-CO-1, LS174T, H1299 and H358, HepG2, PC3, THP-1, Daudi, Jurkat and MDA-MB-231 cells
Concentration:	0-100 μM
Incubation Time:	24 or 72 hours
Result:	Was efficient to kill these tumor cells with an IC₅₀ in the range of 0.42 μM (Hep2G cells) to 7.75 μM (SaOS-2 cells).

体内研究
(In Vivo)

ZZW-115 hydrochloride (0.5-5 mg/kg; injection; daily for 30 days) inhibits the growth of pancreatic xenografted tumors.
ZZW-115 hydrochloride (5 mg/kg for 30 days; immunocompetent C57BL/6 mice were orthotopically implanted with Panc02 cells) treatment shows the tumor size is almost unmeasurable in some cases.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NMRI-Foxn1nu/Foxn1nu mice (nude mice) xenografted with MiaPaCa-2 cells
Dosage:	5, 2.5, 1.0, or 0.5 mg/kg
Administration:	Injection; daily for 30 days
Result:	When the mice were injected with 5 mg/kg ZZW-115 hydrochloride, the tumors stopped growing a few days after treatment and their size decreased progressively, almost disappearing at the end of the treatment.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NMRI-Foxn1nu/Foxn1nu mice (nude mice) xenografted with MiaPaCa-2 cells
Dosage:	5, 2.5, 1.0, or 0.5 mg/kg
Administration:	Injection; daily for 30 days
Result:	When the mice were injected with 5 mg/kg ZZW-115 hydrochloride, the tumors stopped growing a few days after treatment and their size decreased progressively, almost disappearing at the end of the treatment.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NMRI-Foxn1nu/Foxn1nu mice (nude mice) xenografted with MiaPaCa-2 cells
Dosage:	5, 2.5, 1.0, or 0.5 mg/kg
Administration:	Injection; daily for 30 days
Result:	When the mice were injected with 5 mg/kg ZZW-115 hydrochloride, the tumors stopped growing a few days after treatment and their size decreased progressively, almost disappearing at the end of the treatment.

性状

Oil

溶解性数据

In Vitro:

DMSO : 83.33 mg/mL (145.18 mM; Need ultrasonic)

H₂O : 60 mg/mL (104.54 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7423 mL	8.7113 mL	17.4225 mL
5 mM	0.3485 mL	1.7423 mL	3.4845 mL
10 mM	0.1742 mL	0.8711 mL	1.7423 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 50 mg/mL (87.11 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 6.25 mg/mL (10.89 mM); Clear solution

此方案可获得 ≥ 6.25 mg/mL (10.89 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 62.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 6.25 mg/mL (10.89 mM); Clear solution

此方案可获得 ≥ 6.25 mg/mL (10.89 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 62.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明