DIM-C-pPhOH|cas:151358-47-3|西域试剂

DIM-C-pPhOH,99.05%

产品编号：Bellancom-112055| CAS NO：151358-47-3| 分子式：C₂₃H₁₈N₂O| 分子量：338.40

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货号	价格	库存与货期
Bellancom-112055	￥500.00	杭州北京（现货）
Bellancom-112055	￥750.00	杭州北京（现货）
Bellancom-112055	￥950.00	杭州北京（现货）
Bellancom-112055	￥1900.00	杭州北京（现货）
Bellancom-112055	￥2900.00	杭州北京（现货）

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DIM-C-pPhOH

产品介绍

DIM-C-pPhOH 是核受体 4A1 (NR4A1) 拮抗剂。DIM-C-pPhOH 抑制癌细胞的生长和 mTOR 信号传导，诱导细胞凋亡和细胞应激。DIM-C-pPhOH 可降低细胞增殖，对 ACHN 细胞和 786-O 细胞的 IC50 值分别为 13.6 μM 和 13.0 μM。

生物活性

DIM-C-pPhOH is a nuclear receptor 4A1 (NR4A1) antagonist. DIM-C-pPhOH inhibits cancer cell growth and mTOR signaling, induce apoptosis and cellular stress. DIM-C-pPhOH reduces cell proliferation with IC50 values of 13.6 μM and 13.0 μM for ACHN cells and 786-O cells, respectively.

体外研究

DIM-C-pPhOH (7.5-20 μM; 24 hours; ACHN and 786-O cells) treatment significantly decreases cell proliferation.
DIM-C-pPhOH (20 μM; 24 hours; ACHN and 786-O cells) treatment induces Annexin V staining in ACHN and 786-O cells, confirming that DIM-C-pPhOH induce apoptosis, and also induces cleavage of caspases 7 and 8.
DIM-C-pPhOH (15-20 μM; 24 hours; ACHN and 786-O cells) treatment inhibits NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induces sestrin 2, activates AMPKα and inhibits activation of mTOR and downstream kinases.
DIM-C-pPhOH decreases expression of β1-integrin protein and mRNA and β1-integrin-dependent responses in MCF7, MDA-MB-231, and SKBR3 cells and also inhibits migration of the latter two cell lines.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	ACHN and 786-O cells
Concentration:	7.5 μM, 15 μM, 20 μM
Incubation Time:	24 hours
Result:	Significantly decreased cell proliferation.

Apoptosis Analysis

Cell Line:	ACHN and 786-O cells
Concentration:	20 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in ACHN and 786-O cells.

Western Blot Analysis

Cell Line:	ACHN and 786-O cells
Concentration:	15 μM, 20 μM
Incubation Time:	24 hours
Result:	Inhibited NR4A1-regulated expression of survivin, bcl-2 and EGFR in ACHN and 786-O cells. And also induced sestrin 2, activated AMPKα and inhibited activation of mTOR and downstream kinases.

体内研究
(In Vivo)

DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic nude mice (aged 6-7 weeks) injected with ACHN cells
Dosage:	30 mg/kg/day
Administration:	Oral gavage; daily; for 50 days
Result:	Resulted in a significant inhibition of tumor growth.

体内研究

DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic nude mice (aged 6-7 weeks) injected with ACHN cells
Dosage:	30 mg/kg/day
Administration:	Oral gavage; daily; for 50 days
Result:	Resulted in a significant inhibition of tumor growth.

体内研究

DIM-C-pPhOH (30 mg/kg; oral gavage; daily; for 50 days; male athymic nude mice) treatment results in a significant inhibition of tumor growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male athymic nude mice (aged 6-7 weeks) injected with ACHN cells
Dosage:	30 mg/kg/day
Administration:	Oral gavage; daily; for 50 days
Result:	Resulted in a significant inhibition of tumor growth.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (369.39 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9551 mL	14.7754 mL	29.5508 mL
5 mM	0.5910 mL	2.9551 mL	5.9102 mL
10 mM	0.2955 mL	1.4775 mL	2.9551 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.15 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.15 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (6.15 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (6.15 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。