Millepachine|cas:1393922-01-4|西域试剂

Millepachine,99.65%

产品编号：Bellancom-N7591| CAS NO：1393922-01-4| 分子式：C₂₂H₂₂O₄| 分子量：350.41

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-N7591	￥2500.00	杭州北京（现货）
Bellancom-N7591	￥4000.00	杭州北京（现货）
Bellancom-N7591	￥14000.00	杭州北京（现货）
Bellancom-N7591	￥22400.00	杭州北京（现货）

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Millepachine

产品介绍

Millepachine 是来自中草药 Millettia pachycarpa Benth 的生物活性天然查尔酮，在体内外对多种人类癌细胞均显示出强大的抗肿瘤作用。

生物活性

Millepachine is a bioactive natural chalcone from Chinese herbal medicine Millettia pachycarpa Benth, exhibits strong antitumor effects against numerous human cancer cells both in vitro and in vivo.

体外研究

Millepachine (1.25-20 μM; 48 h) remarkably inhibits the proliferation of cisplatin-resistant A2780CP cells.
Millepachine (2-8 μM; 24 or 48 h) induces G2/M arrest and apoptosis cisplatin-sensitive A2780S and cisplatin-resistant A2780CP cells.
Millepachine (2-8 μM; 24 h) decreases topoisomerase II levels in A2780S and A2780CP cells.
Millepachine (2-8 μM; 24 h) inhibits ATP-binding cassette transporter activity in A2780CP cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	A2780CP cells
Concentration:	0, 1.25, 2.5, 5, 10, 20 μM
Incubation Time:	48 hours
Result:	Inhibited the cells proliferation with an IC₅₀ of 4 μM.

Cell Cycle Analysis

Cell Line:	A2780S and A2780CP cells
Concentration:	2, 4, 8 μM
Incubation Time:	24 or 48 hours
Result:	Induced significant G2/M arrest both in both cells. The percentage of cells in the G2/M fraction increased from 15.99% in vehicle cells to 24.93%, 60.67%, and 77.31% at dose of 2, 4, and 8 μM in A2780S cells, respectively.

Apoptosis Analysis

Cell Line:	A2780S and A2780CP cells
Concentration:	2, 4, 8 μM
Incubation Time:	24 or 48 hours
Result:	The percentage of apoptotic cells increased from 1.49% in vehicle cells to 10.98%, 20.60%, and 39.43% at dose of 2, 4, and 8 μM in A2780S cells, respectively. The percentage of apoptotic cells increased from 0.87% to 10.97%, 25.28%, and 37.59% in A2780CP cells, respectively.

Western Blot Analysis

Cell Line:	A2780S and A2780CP cells
Concentration:	0, 2, 4, 8 μM
Incubation Time:	24 hours
Result:	Decreased the levels of topoisomerase II (TOPO II) in both cells.

体内研究
(In Vivo)

Millepachine (20 mg/kg; i.v. every two days for 14 day) inhibits tumor growth in mice.
Millepachine (20 mg/kg; i.v. every two days for 14 day) does not induce acquired drug resistance in an excised A2780S xenograft model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells
Dosage:	20 mg/kg
Administration:	I.v. every two days for 2-14 days
Result:	Reduced tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively. With low toxicity in vivo.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells
Dosage:	20 mg/kg
Administration:	I.v. every two days for 2-14 days
Result:	Reduced tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively. With low toxicity in vivo.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells
Dosage:	20 mg/kg
Administration:	I.v. every two days for 2-14 days
Result:	Reduced tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively. With low toxicity in vivo.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (285.38 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8538 mL	14.2690 mL	28.5380 mL
5 mM	0.5708 mL	2.8538 mL	5.7076 mL
10 mM	0.2854 mL	1.4269 mL	2.8538 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.5 mg/mL (7.13 mM); Suspended solution; Need ultrasonic

此方案可获得 2.5 mg/mL (7.13 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.13 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.13 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。