CCG-1423|cas:285986-88-1|西域试剂

CCG-1423,99.94%

产品编号：Bellancom-13991| CAS NO：285986-88-1| 分子式：C₁₈H₁₃ClF₆N₂O₃| 分子量：454.75

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-13991	￥650.00	杭州北京（现货）
Bellancom-13991	￥1850.00	杭州北京（现货）
Bellancom-13991	￥3100.00	杭州北京（现货）
Bellancom-13991	￥4850.00	杭州北京（现货）

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CCG-1423

产品介绍

CCG-1423 是 Rho/MRTF/SRF 通路的抑制剂。CCG-1423 在多种癌细胞中具有活性。CCG-1423 是开发新型活性分子工具的先导化合物，可用于癌症和糖尿病的研究。

生物活性

CCG-1423 is an inhibitor of Rho/MRTF/SRF pathway. CCG-1423 shows activities in several cancer cells. CCG-1423 is a promising lead compound for the development of novel pharmacologic tools, and it can be used for the research of cancer and diabetes.

体外研究

CCG-1423 (10 µM; 24 h) affects invasion by cultured PC-3 cells into a Matrigel matrix and inhibits 54% mitochondrial metabolism of WST-1.
CCG-1423 (0-100 µM; 24 h) inhibits RhoA and RhoC signaling pathways with an IC₅₀ value of 1.5 µM for Rho-pathway selective serum response element-luciferase reporter.
CCG-1423 (1 µM; 16 h) improves glucose uptake in both L6 cells and primary human myotubes.
CCG-1423 (10 µM; 18-19 h) inhibits expression of Rho downstream.
CCG-1423 (3 µM; 25 h) selectively stimulates apoptosis of RhoC-overexpressing melanoma cell line (A375M2) compared with the parental cell line (A375).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Invasion Assay

Cell Line:	PC-3 cell line
Concentration:	10 µM
Incubation Time:	24 hours
Result:	Inhibited 71% invasion by cultured PC-3 cells into a Matrigel matrix.

Western Blot Analysis

Cell Line:	L6 myotubes
Concentration:	1 µM
Incubation Time:	48 hours
Result:	Increased insulin-stimulated Akt phosphorylation, blocked ERK phosphorylation, and increased IRS-1 tyrosine phosphorylation and its association with the p85 regulatory subunit of PI3K.

Cell Proliferation Assay

Cell Line:	PC-3 cell line
Concentration:	0.3 µM
Incubation Time:	8 days
Result:	Inhibited growth of PC-3 prostate cancer cells with an IC₅₀ value of 1 µM with 30 µM LPA adding.

Apoptosis Analysis

Cell Line:	RhoC-overexpressing A375M2 and low RhoC-expressing A375 melanoma cell lines
Concentration:	3 µM
Incubation Time:	25 hours
Result:	Stimulated apoptosis of A375M2 cell line compared with the parental cell line.

体内研究
(In Vivo)

CCG-1423 (0.15 mg/kg; i.p. once daily for two weeks) affects glucose tolerance and insulin levels in HFD-fed mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	16-week-old mice with HFD-induced obesity
Dosage:	0.15 mg/kg
Administration:	Intraperitoneal injection; 0.15 mg/kg once per day; for two weeks
Result:	Improved glucose tolerance and reduced insulin levels at 30 minutes after glucose injection.

体内研究

CCG-1423 (0.15 mg/kg; i.p. once daily for two weeks) affects glucose tolerance and insulin levels in HFD-fed mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	16-week-old mice with HFD-induced obesity
Dosage:	0.15 mg/kg
Administration:	Intraperitoneal injection; 0.15 mg/kg once per day; for two weeks
Result:	Improved glucose tolerance and reduced insulin levels at 30 minutes after glucose injection.

体内研究

CCG-1423 (0.15 mg/kg; i.p. once daily for two weeks) affects glucose tolerance and insulin levels in HFD-fed mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	16-week-old mice with HFD-induced obesity
Dosage:	0.15 mg/kg
Administration:	Intraperitoneal injection; 0.15 mg/kg once per day; for two weeks
Result:	Improved glucose tolerance and reduced insulin levels at 30 minutes after glucose injection.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (219.90 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1990 mL	10.9951 mL	21.9901 mL
5 mM	0.4398 mL	2.1990 mL	4.3980 mL
10 mM	0.2199 mL	1.0995 mL	2.1990 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.50 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.50 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。