Pladienolide B|cas:445493-23-2|西域试剂

Pladienolide B

产品编号：Bellancom-16399| CAS NO：445493-23-2| 分子式：C₃₀H₄₈O₈| 分子量：536.70

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-16399		￥5500.00	杭州北京（现货）

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Pladienolide B

产品介绍

Pladienolide B 是一种有效的癌细胞生长抑制剂，其作用靶向剪接体的 SF3B1 亚单位。Pladienolide B 通过抑制前 mRNA 剪接发挥抗肿瘤作用，诱导调亡。

生物活性

Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3B1 subunit of the spliceosome. Pladienolide B exerts antitumor activities mediated through the inhibition of pre-mRNA splicing. Pladienolide B induces apoptosis.

体外研究

Pladienolide B (0.1-2 nM; 24-72 hours) inhibits human cervical carcinoma cells viability.
Pladienolide B (0.1-2 nM; 24-48 hours) reduces SF3b1 expression in human cervical carcinoma cells.
Pladienolide B induces (0.1-2 nM; 24 hours) cell cycle arrest and apoptosis.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	HeLa cells
Concentration:	0.1, 0.5, 1, 1.5, 2 nM
Incubation Time:	24, 48, 72 hours
Result:	Significantly decreased cell viability, and the decrease was concentration- and time-dependent.

Apoptosis Analysis

Cell Line:	HeLa cells
Concentration:	0.1, 0.5, and 2 nM
Incubation Time:	24 hours
Result:	The apoptotic cells were highly induced at 24 hours.

RT-PCR

Cell Line:	HeLa cells
Concentration:	0.1, 0.5, and 2 nM
Incubation Time:	24, 48 hours
Result:	Induced a time- and concentration-dependent decrease in cellular SF3b1 proteins.

体内研究
(In Vivo)

Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days) has strong antitumor activities^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female or male BALB/c nu/nu mice (7 weeks of age) (PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts)^[4]
Dosage:	2.5, 5, and 10 mg/kg
Administration:	I.v.; daily for 5 days
Result:	Showed strong growth inhibitory or regressive activities against these xenografts.

体内研究

Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days) has strong antitumor activities^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female or male BALB/c nu/nu mice (7 weeks of age) (PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts)^[4]
Dosage:	2.5, 5, and 10 mg/kg
Administration:	I.v.; daily for 5 days
Result:	Showed strong growth inhibitory or regressive activities against these xenografts.

体内研究

Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days) has strong antitumor activities^[4].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female or male BALB/c nu/nu mice (7 weeks of age) (PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts)^[4]
Dosage:	2.5, 5, and 10 mg/kg
Administration:	I.v.; daily for 5 days
Result:	Showed strong growth inhibitory or regressive activities against these xenografts.