Mirin|cas:299953-00-7|西域试剂

Mirin,98.02%

产品编号：Bellancom-117693| CAS NO：299953-00-7| 分子式：C₁₀H₈N₂O₂S| 分子量：220.25

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货号	价格	库存与货期
Bellancom-117693	￥500.00	杭州北京（现货）
Bellancom-117693	￥800.00	杭州北京（现货）
Bellancom-117693	￥2400.00	杭州北京（现货）
Bellancom-117693	￥3900.00	杭州北京（现货）

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Mirin

产品介绍

Mirin 一种有效的 Mre11-Rad50-Nbs1 (MRN) 复合物抑制剂。Mirin 在不影响 ATM 蛋白激酶活性的情况下阻止 ATM (ataxia-telangiectasia mutated) 的 MRN 依赖性激活 (IC₅₀=12 μM)，并抑制 Mre11 相关的核酸外切酶活性。Mirin 可消除哺乳动物细胞中的 G2/M 检查点和同源性依赖性修复。Mirin 在哺乳动物细胞中阻止 ATM 因 DNA 双链断裂 (DSBs) 而激活并阻断同源性定向修复 (HDR)。

生物活性

Mirin is a potent Mre11-Rad50-Nbs1 (MRN) complex inhibitor. Mirin prevents MRN-dependent activation of ATM (IC₅₀=12 μM) without affecting ATM protein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin prevents ATM activation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells.

体外研究

Mirin inhibits H2AX phosphorylation with an IC₅₀ of 66 μM. Mirin also inhibits the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. Mirin induces a substantial G2 arrest at concentrations of 50 μM and 100 μM. Mirin (10-100 μM) inhibits homology-dependent DNA repair in TOSA4 cells.
BRCA2-deficient cells also showed hypersensitivity to the Mre11 inhibitor Mirin.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 83.33 mg/mL (378.34 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.5403 mL	22.7015 mL	45.4030 mL
5 mM	0.9081 mL	4.5403 mL	9.0806 mL
10 mM	0.4540 mL	2.2701 mL	4.5403 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

参考文献