Ginkgetin 银杏双黄酮|cas:481-46-9|西域试剂

Ginkgetin 银杏双黄酮,99.86%

产品编号：Bellancom-N0889| CAS NO：481-46-9| 分子式：C₃₂H₂₂O₁₀| 分子量：566.51

银杏双黄酮(Ginkgetin)是一种天然的双类黄酮，从银杏叶中分离，抗炎和抗癌生物活性。

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货号	价格	库存与货期
Bellancom-N0889	￥1500.00	杭州北京（现货）
Bellancom-N0889	￥2400.00	杭州北京（现货）
Bellancom-N0889	￥5300.00	杭州北京（现货）

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Ginkgetin 银杏双黄酮

产品介绍

Ginkgetin 是一种从银杏叶中分离得到的双黄酮。Ginkgetin 具有抗肿瘤，抗炎，神经保护，抗真菌的作用。Ginkgetin 也是一种有效的 Wnt 信号抑制剂，IC₅₀ 值为 5.92 μM。

生物活性

Ginkgetin, a biflavone, is isolated from Ginkgo biloba leaves. Ginkgetin exhibit anti-tumor, anti-inflammatory, neuroprotective, anti-fungal activities. Ginkgetin is also a potent inhibitor of Wnt signaling, with an IC₅₀ of 5.92 μΜ^[4]^[5].

体外研究

Ginkgetin (2.5-20 μM; 48 h) inhibits the growth of Daoy and D283 cell lines, and induces G₂/M cell cycle arrest in Daoy cells.
Ginkgetin (20-40 μM; 24 h) significantly activates the apoptosis of osteosarcoma cells in a concentration-dependent manner.
Ginkgetin (10-20 μM; 3-24 h) down-regulated the expression of Wnt target genes without affecting the expression of β-catenin in medulloblastoma cells.
Ginkgetin (1-10 μM; 24 or 48 h) significantly inhibits the VEGF-induced endothelial cell proliferation, migration, and wound recovery in a concentration-dependent manner.
Ginkgetin (5-10 μM; 48 h) induces autophagy responsible for cell death in A549^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Daoy and D283 cell lines
Concentration:	2.5, 5, 10, 20 μM
Incubation Time:	48 hours
Result:	Inhibited the cell growth, with IC₅₀s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively.

Apoptosis Analysis

Cell Line:	Osteosarcoma cells
Concentration:	20, 30, 40 μM
Incubation Time:	24 hours
Result:	Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner.

Cell Cycle Analysis

Cell Line:	Daoy cells
Concentration:	2.5, 5, 10, 20 μM
Incubation Time:	24 hours
Result:	Increased G₂/M phase, compared with that of control, indicating a G₂/M cell phase arrest.

Cell Cycle Analysis

Cell Line:	Daoy and D283 cell lines
Concentration:	10, 20 μM
Incubation Time:	3, 6, 12, 24 hours
Result:	Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells. Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner.

体内研究
(In Vivo)

Ginkgetin (25-100 mg/kg; i.p. 2 hours after the onset of ischemia) exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway^[4].
Ginkgetin (30 mg/kg; intragastrically once per day for 42 d) suppresses tumor growth in A549 cells bearing nude mice^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (200-220 g)^[4]
Dosage:	25, 50, 100 mg/kg
Administration:	I.p. 2 hours after the onset of ischemia
Result:	Reduced the neurological deﬁcit score. Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα. Decreased the expressions of ICAM-1, COX-2, and iNOS. Downregulated downstream inﬂammatory factor PGE2 and TNF-α expression. Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (200-220 g)^[4]
Dosage:	25, 50, 100 mg/kg
Administration:	I.p. 2 hours after the onset of ischemia
Result:	Reduced the neurological deﬁcit score. Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα. Decreased the expressions of ICAM-1, COX-2, and iNOS. Downregulated downstream inﬂammatory factor PGE2 and TNF-α expression. Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (200-220 g)^[4]
Dosage:	25, 50, 100 mg/kg
Administration:	I.p. 2 hours after the onset of ischemia
Result:	Reduced the neurological deﬁcit score. Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα. Decreased the expressions of ICAM-1, COX-2, and iNOS. Downregulated downstream inﬂammatory factor PGE2 and TNF-α expression. Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 20.83 mg/mL (36.77 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7652 mL	8.8260 mL	17.6519 mL
5 mM	0.3530 mL	1.7652 mL	3.5304 mL
10 mM	0.1765 mL	0.8826 mL	1.7652 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 0.5% CMC-Na/saline water
Solubility: 2 mg/mL (3.53 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C
2.

请依序添加每种溶剂： 50% PEG300 50% saline
Solubility: 2 mg/mL (3.53 mM); Suspended solution; Need ultrasonic and warming

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

参考文献

. Hu WH, et, al. Synergy of Ginkgetin and Resveratrol in Suppressing VEGF-Induced Angiogenesis: A Therapy in Treating Colorectal Cancer. Cancers (Basel). 2019 Nov 20;11(12):1828. [Content Brief]

. Ye ZN, et, al. Biflavone Ginkgetin, a Novel Wnt Inhibitor, Suppresses the Growth of Medulloblastoma. Nat Prod Bioprospect. 2015 Mar 29;5(2):91-97. [Content Brief]

. Xiong M, et, al. Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncol Rep. 2016 Feb;35(2):1034-40. [Content Brief]

[4]. Li Q, et, al. Ginkgetin exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway. Biosci Biotechnol Biochem. 2019 Apr;83(4):675-683. [Content Brief]

[5]. Lou J, et, al. Ginkgetin induces autophagic cell death through p62/SQSTM1-mediated autolysosome formation and redox setting in non-small cell lung cancer. Oncotarget. 2017 Oct 16;8(54):93131-93148. [Content Brief]

化学品安全说明书(MSDS)

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质检证书(COA)

产品编号(Item Number)

批号(Lot Number)

1. 物质的识别

产品名：	Ginkgetin
CAS号：	481-46-9
制造商/供应商：	西域试剂网站：www.hzbp.cn 邮件：13911702513@139.com

2. 合成/成分数据

产品名：	Ginkgetin
分子式：	C32H22O10
分子量：	566.51

3. 急救措施

吸入后：	如果吸入，移至空气新鲜处，如果呼吸困难，给输氧，如呼吸停止，给予人工呼吸。
皮肤接触后：	用大量的水冲洗，移除污染的衣服和鞋子。
眼睛接触后：	检查并取下隐形眼镜，并用大量的水冲洗；呼叫医生。
吞食后：	如果吞食，用大量纯净水漱口；呼叫医生。

4. 消防措施

适当的灭火剂：	雾状水，二氧化碳，干粉或泡沫。
防护设备：	穿戴自给式呼吸器和防护服，以防止与皮肤和眼睛接触。

5. 泄漏应急处理

安全防范措施：	封锁泄漏区域；穿戴自给式呼吸器，防护服和厚橡胶手套。
清洁/收集措施：	使用液体粘合原料（硅藻土，通用粘合剂）吸取精细粉末；使用酒精擦洗表面和设备除去污渍；根据第11条处理被污染的材料。

6. 处理和储存

安全处理说明：	避免吸入和接触皮肤，眼睛及衣物；材料可能略微具有刺激性。
储存：	2-8°C, dry, sealed

7. 接触控制和个人防护

呼吸设备：	NIOSH / MSHA认可的呼吸器。
双手保护：	耐化学腐蚀的橡胶手套。
眼睛防护：	化学安全护目镜。

8. 稳定性和反应活性

稳定性：	按照说明存储是稳定的；避免强氧化剂。
热分解/其他要避免的情况：	避免光和热。

9. 毒性资料

急性毒性：	无可用资料。
主要刺激性影响：	无可用资料。
在皮肤上：	无可用资料。
对眼睛：	无可用资料；可能具有刺激性。