LDCA|cas:349106-80-5|西域试剂

LDCA,99.18%

产品编号：Bellancom-147361| CAS NO：349106-80-5| 分子式：C₈H₅Cl₃FNO| 分子量：256.49

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-147361	￥800.00	杭州北京（现货）
Bellancom-147361	￥1300.00	杭州北京（现货）
Bellancom-147361	￥2500.00	杭州北京（现货）
Bellancom-147361	￥4000.00	杭州北京（现货）
Bellancom-147361	￥6500.00	杭州北京（现货）

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LDCA

产品介绍

LDCA 是一种双重打击代谢调节剂，可抑制 LDH-A 酶活性，刺激恶性群体的凋亡。LDCA 可用于致癌进展的研究。

生物活性

LDCA is a dual-hit metabolic modulator and inhibits LDH-A enzyme activity to stimulate apoptosis in the malignant population. LDCA can be used for the research of oncogenic progression.

体外研究

LDCA (2-100 μM; 16-72 h) is used in combination with doxorubicin synergistically, enhances the growth inhibition and induces mitochondria-mediated apoptosis by recruiting the caspase cascade, restricting migration, and obviating the clonogenic outgrowth potential of melanoma cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	B16-F10 cells
Concentration:	2-100 μM
Incubation Time:	72 h
Result:	Arrested cell growth with a dose-dependent cytotoxic effect and had a strong synergism with LDCA.

Apoptosis Analysis

Cell Line:	B16-F10 cells
Concentration:	20 μM
Incubation Time:	24 h
Result:	Resulted 15% death when cells exposed to LDCA and caused 40% melanoma cell death combination synergistically with doxorubicin.

Immunofluorescence

Cell Line:	B16-F10 cells
Concentration:	20 μM
Incubation Time:	16 h
Result:	Demonstrated that combination with doxorubicin resultantly affected cellular morphology with condensed and fragmented nuclei.

Cell Migration Assay

Cell Line:	B16-F10 cells
Concentration:	20 μM
Incubation Time:	16 h
Result:	Signicantly limited the migratory potential in the B16-F10 cells.

体内研究
(In Vivo)

LDCA (2 mg/kg, iv., the 6th day, once) is used in combination with doxorubicin thwarts tumor growth kinetics to restrain oncogenic progression, thus accentuating survival in the model of murine melanoma.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	melanoma tumor model
Dosage:	2 mg/kg
Administration:	2 mg/kg, iv., the 6th day, once
Result:	Significantly increased mice survivability combination with doxorubicin, and relieved mice tumor necrosis phenomena.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	melanoma tumor model
Dosage:	2 mg/kg
Administration:	2 mg/kg, iv., the 6th day, once
Result:	Significantly increased mice survivability combination with doxorubicin, and relieved mice tumor necrosis phenomena.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	melanoma tumor model
Dosage:	2 mg/kg
Administration:	2 mg/kg, iv., the 6th day, once
Result:	Significantly increased mice survivability combination with doxorubicin, and relieved mice tumor necrosis phenomena.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (389.88 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.8988 mL	19.4939 mL	38.9879 mL
5 mM	0.7798 mL	3.8988 mL	7.7976 mL
10 mM	0.3899 mL	1.9494 mL	3.8988 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (9.75 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.75 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.75 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.75 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.75 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.75 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。