8pyDTZ|cas:2351898-91-2|西域试剂

8pyDTZ,98.0%

产品编号：Bellancom-135368| CAS NO：2351898-91-2| 分子式：C₂₄H₁₈N₄O| 分子量：378.43

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	包装	价格	库存与货期	购买量	操作
Bellancom-135368		￥4500.00	杭州北京（现货）
Bellancom-135368		￥6800.00	杭州北京（现货）

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8pyDTZ

产品介绍

8pyDTZ 是一种吡啶基二苯基叔嗪 (DTZ) 类似物，也是一种 LumiLuc 荧光素酶的 ATP 非依赖性吡啶基底物。8pyDTZ 具有出色的生物相容性和体内敏感性，可用于生物体内发光成像。

生物活性

8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission. 8pyDTZ has excellent biocompatibility and superior in vivo sensitivity. 8pyDTZ can be used for in vivo luminescence imaging.

体外研究

Compared to DTZ, 8pyDTZ (compounds 3c) is able to emit red-shifted chemiluminescence or bioluminescence. 8pyDTZ shows the most red-shifted emission and red-shifted photons can penetrate through tissue better.
LumiLuc-8pyDTZ in human embryonic kidney (HEK) 293T cells transiently expressing the luciferase is evaluated. The LumiLuc-8pyDTZ pair produces ∼3-5-fold more bioluminescence than teLuc-8pyDTZ. To compare far-red emission intensities of bioluminescent reporters, HEK 293T cells in the presence of a 600-700 nm band pass filter are imaged. At substrate concentrations from 6.25 to 100 μM, LumiLuc-8pyDTZ consistently produces 1.6-3.9-fold higher photon flux than teLuc-DTZ. No ATP perturbation is observed from 8pyDTZ-treated, LumiLucexpressing cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

LumiLuc-8pyDTZ pair is compared with several benchmark reporters in a tumor xenograft mouse model. LumiLuc-8pyDTZ shows high sensitivity and does not need organic cosolvents for in vivo administration. LumiScarlet-8pyDTZ is comparable to Akaluc-AkaLumine, the brightest ATP-dependent luciferase-luciferin pair, for detecting cells in deep tissues of mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

EtOH+HCl : 2 mg/mL (5.28 mM; ultrasonic and adjust pH to 2 with 1M HCl; DMSO can inactivate 8pyDTZ's activity)

DMF : 1 mg/mL (2.64 mM; Need ultrasonic; DMSO can inactivate 8pyDTZ's activity)

H₂O : < 0.1 mg/mL (insoluble; DMSO can inactivate 8pyDTZ's activity)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.6425 mL	13.2125 mL	26.4250 mL
5 mM	0.5285 mL	2.6425 mL	5.2850 mL
10 mM	---	---	---

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

参考文献

. Yeh HW, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965. [Content Brief]

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