C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells. Storage: protect from light.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

荧光衰老相关的 β-半乳糖苷酶 (SA-β-Gal) 检测:
1. 在 6、12、24 或 96 孔板中以 5×10⁵ 细胞/mL 的密度培养细胞过夜。或根据您的常规方案孵育细胞。
2. 用 200 μL PBS 洗涤细胞一次，并用 100 μL 固定液 (2% 甲醛/0.2% 戊二醛蒸馏水) 室温固定 5 分钟。
3. 用 200 μL PBS 洗涤细胞两次，用 100 μL 33 μM C12FDG (PBS，pH=6.0) 染色 10 分钟，然后用在在 200 μL Hoechst 溶液 (PBS，pH=6.0) (例如 1 μg/mL Hoechst 33342，HY-15559) 染色 10 分钟。
4. 用 20× 物镜和 360-nm (Hoechst 33342) 和 480-nm (C12FDG) 激发滤光片对这些细胞成像，并分别通过 460-nm 和 535-nm 发射滤光片进行监测。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41.  [Content Brief]

  . Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8.  [Content Brief]