Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling diazo-labeled proteins:
1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature.
2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 °C for 18 h.
3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with λ_ex=365 nm, λ_em=480 nm.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Ke Wang, et al. A metal-free turn-on fluorescent probe for the fast and sensitive detection of inorganic azides. Bioorg Med Chem Lett. 2016 Apr 1;26(7):1651-4.  [Content Brief]

  . Frédéric Friscourt, et al. Fluorogenic Strain-Promoted Alkyne-Diazo Cycloadditions. Chemistry. 2015 Sep 28;21(40):13996-4001. doi: 10.1002/chem.201502242. Epub 2015 Aug 18.  [Content Brief]

  . Camille Favre, et al. Sydnone Reporters for Highly Fluorogenic Copper-Free Click Ligations. J Org Chem. 2018 Feb 16;83(4):2058-2066.  [Content Brief]