Radioprotectin-1|cas:1622006-09-0|西域试剂

Radioprotectin-1,99.51%

产品编号：Bellancom-114380| CAS NO：1622006-09-0| 分子式：C₂₃H₁₉ClN₂O₆S| 分子量：486.92

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货号	价格	库存与货期
Bellancom-114380	￥4500.00	杭州北京（现货）
Bellancom-114380	￥7500.00	杭州北京（现货）
Bellancom-114380	￥22000.00	杭州北京（现货）
Bellancom-114380	￥33000.00	杭州北京（现货）

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Radioprotectin-1

产品介绍

Radioprotectin-1 是一种高效特异性的溶血磷脂酸受体 2 (LPA₂) 激动剂，EC₅₀ 值为 25 nM (鼠源 LPA₂ 亚型) 。

生物活性

Radioprotectin-1 is a potent and specific nonlipid agonist of lysophosphatidic acid receptor 2 (LPA₂), with an EC₅₀ value of 25 nM for murine LPA₂ subtype.

体外研究

Radioprotectin-1 is a potent agonist of LPA₂ with an EC₅₀ of 5 pM and functions as a full agonist at the human ortholog of LPA₂.
Radioprotectin-1 (0-3 μM; 15 minutes) effectively reduces apoptosis induced by γ-irradiation and the radiomimetic drug Adriamycin in cells that expressed LPA₂ either endogenously or after transfection.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	MEF cells , IEC-6 cells
Concentration:	0 μM, 0.1 μM, 0.3 μM, 1.0 μM,3 μM
Incubation Time:	15 minutes
Result:	Protected cells from apoptosis by γ-irradiation- and the radiomimetic chemotherapeutic Adiamycin.

体内研究
(In Vivo)

Radioprotectin-1 is a high-potency specific agonist of the murine LPA₂ GPCR .
Radioprotectin-1 (0.1 mg/kg, 0.3 mg/kg; s.c.; every 12 hours; for 3 days) decreases the mortality of C57BL/6 mice in models of the hematopoietic acute radiation syndromes (HE-ARS) and gastrointestinal acute radiation syndromes (GI-ARS) .
Radioprotectin-1 exerts its radioprotective and radiomitigative action through specific activation of the upregulated LPA₂ GPCR in Lgr5⁺stem cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-10 weeks old C57BL/6 female mice bearing total body irradiation (TBI)
Dosage:	0.1 mg/kg, 0.3 mg/kg
Administration:	Subcutaneous injection; every 12 hours; for 3 days
Result:	Decreased mortality of C57BL/6 mice in models of the HE-ARS and the GI-ARS.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-10 weeks old C57BL/6 female mice bearing total body irradiation (TBI)
Dosage:	0.1 mg/kg, 0.3 mg/kg
Administration:	Subcutaneous injection; every 12 hours; for 3 days
Result:	Decreased mortality of C57BL/6 mice in models of the HE-ARS and the GI-ARS.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-10 weeks old C57BL/6 female mice bearing total body irradiation (TBI)
Dosage:	0.1 mg/kg, 0.3 mg/kg
Administration:	Subcutaneous injection; every 12 hours; for 3 days
Result:	Decreased mortality of C57BL/6 mice in models of the HE-ARS and the GI-ARS.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (256.72 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.0537 mL	10.2686 mL	20.5373 mL
5 mM	0.4107 mL	2.0537 mL	4.1075 mL
10 mM	0.2054 mL	1.0269 mL	2.0537 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.27 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.27 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.27 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。