YC-001|cas:748778-73-6|西域试剂

YC-001,99.47%

产品编号：Bellancom-124717| CAS NO：748778-73-6| 分子式：C₁₂H₇ClO₂S₂| 分子量：282.77

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-124717	￥900.00	杭州北京（现货）
Bellancom-124717	￥1500.00	杭州北京（现货）
Bellancom-124717	￥3000.00	杭州北京（现货）
Bellancom-124717	￥4800.00	杭州北京（现货）
Bellancom-124717	￥7200.00	杭州北京（现货）

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YC-001

产品介绍

YC-001 是杆状视蛋白 (rod opsin) 的反向激动剂和拮抗剂。YC-001 可逆地结合视杆视蛋白并稳定视杆视蛋白的结构。YC-001 保护小鼠免受强光诱导的视网膜变性。YC-001 具有研究视网膜变性的潜力。

生物活性

YC-001 is an inverse agonist and antagonist of rod opsin. YC-001 reversibly binds rod opsin and stabilizes the rod opsin structure. YC-001 protects mice from bright light-induced retinal degeneration. YC-001 has the potential for the research of retinal degeneration.

体外研究

YC-001 (0-100 µM) shows a potency activity of 8.7 µM and an efficacy at 150–310%.
YC-001 (0.5, 1, 5, 10, 20, 40 µM) improves the glycosylation profile of P23H opsin mutant.
YC-001 (0,-1.5 µM) reversibly binds rod opsin with EC₅₀ OF 0.98 µM.
YC-001 (0.313, 0.625, 1.25, 2.5, 5, 10, 20, 80 µM) increases in cAMP level in a dose-dependent manner in NIH3T3 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	NIH3T3 cells
Concentration:	0.5, 1, 5, 10, 20, 40 µM
Incubation Time:
Result:	Improved the glycosylation profile of P23H opsin.

体内研究
(In Vivo)

YC-001 (50, 200mg/kg; i.p.) protects Abca4−/−Rdh8−/− mice from bright light-induced retinal degeneration.
YC-001 (200mg/kg; i.p.) enters mouse eyes after systemic administration but is not retained for prolonged periods.
YC-001 (100 mg/kg, 200 mg/kg; i.p.; daily for 24 days) shows virtually no acute toxicity.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-week-old Abca4 −/−Rdh8−/− mice
Dosage:	50, 200mg/kg
Administration:	I.P.
Result:	Protected Abca4−/−Rdh8−/− mice from bright light-induced retinal degeneration.

Animal Model:	C57BL/6 mouse
Dosage:	200 mg/kg
Administration:	I.P.
Result:	Entered mouse eyes after systemic administration but is not retained for prolonged periods.

Animal Model:	C57BL/6 mice
Dosage:	100 mg/kg, 200 mg/kg
Administration:	I.p.; daily, 24 days
Result:	Showed no acute toxicity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-week-old Abca4 −/−Rdh8−/− mice
Dosage:	50, 200mg/kg
Administration:	I.P.
Result:	Protected Abca4−/−Rdh8−/− mice from bright light-induced retinal degeneration.

Animal Model:	C57BL/6 mouse
Dosage:	200 mg/kg
Administration:	I.P.
Result:	Entered mouse eyes after systemic administration but is not retained for prolonged periods.

Animal Model:	C57BL/6 mice
Dosage:	100 mg/kg, 200 mg/kg
Administration:	I.p.; daily, 24 days
Result:	Showed no acute toxicity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-week-old Abca4 −/−Rdh8−/− mice
Dosage:	50, 200mg/kg
Administration:	I.P.
Result:	Protected Abca4−/−Rdh8−/− mice from bright light-induced retinal degeneration.

Animal Model:	C57BL/6 mouse
Dosage:	200 mg/kg
Administration:	I.P.
Result:	Entered mouse eyes after systemic administration but is not retained for prolonged periods.

Animal Model:	C57BL/6 mice
Dosage:	100 mg/kg, 200 mg/kg
Administration:	I.p.; daily, 24 days
Result:	Showed no acute toxicity.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (353.64 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.5364 mL	17.6822 mL	35.3644 mL
5 mM	0.7073 mL	3.5364 mL	7.0729 mL
10 mM	0.3536 mL	1.7682 mL	3.5364 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (8.84 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.84 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (8.84 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.84 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。