AG1024 Tyrphostin AG 1024|cas:65678-07-1|西域试剂

AG1024 Tyrphostin AG 1024,99.82%

产品编号：Bellancom-10253| CAS NO：65678-07-1| 分子式：C₁₄H₁₃BrN₂O| 分子量：305.17

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货号	价格	库存与货期
Bellancom-10253	￥600.00	杭州北京（现货）
Bellancom-10253	￥1100.00	杭州北京（现货）
Bellancom-10253	￥3500.00	杭州北京（现货）
Bellancom-10253	￥6000.00	杭州北京（现货）

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AG1024 Tyrphostin AG 1024

产品介绍

AG1024 (Tyrphostin AG 1024) 是一种可逆的，竞争性和选择性的胰岛素样生长因子-1 受体 (IGF-1R) 抑制剂，IC₅₀ 为 7 μM。AG1024 抑制胰岛素受体 (IR) 的磷酸化 (IC₅₀=57 μM)。AG1024 诱导细胞凋亡 (apoptosis) 并具有抗癌活性。

生物活性

AG1024 (Tyrphostin AG 1024) is a reversible, competitive and selective IGF-1R inhibitor with an IC₅₀ of 7 μM. AG1024 inhibits phosphorylation of IR (IC₅₀=57 μM). AG1024 induces apoptosis and has anti-cancer activity.

体外研究

AG1024 (Tyrphostin AG 1024; 2-10 μM; 1-5 days) shows a dose-dependent inhibition of cell proliferation.
AG1024 (1-5 μM; 1-3 days) induces UT7-9 and Baf3-p210 cells apoptosis.
AG1024 (2 μM; 6, 12 hours) downregulates phospho-Akt, Bcr-Abl and upregulates DNA-PKcs.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	UT7-9 and Baf3-p210 cells
Concentration:	2, 5, 10 μM
Incubation Time:	1, 3, 5 days
Result:	Showed a dose-dependent inhibition of cell proliferation.

Apoptosis Analysis

Cell Line:	UT7-9 and Baf3-p210 cells
Concentration:	1, 3, 5 μM
Incubation Time:	1, 2, 3 days
Result:	Induced apoptosis.

Western Blot Analysis

Cell Line:	UT7-9 and Ba/F3-p210 cells
Concentration:	2 μM
Incubation Time:	6, 12 hours
Result:	Downregulated phospho-Akt, Bcr-Abl and upregulated DNA-PKcs.

体内研究
(In Vivo)

AG1024 (Tyrphostin AG 1024; 30 μg; i.p.; per day; for 2 weeks) significantly delays the tumour growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female nude mice (6-8 weeks old)
Dosage:	30 μg
Administration:	IP; per day; for 2 weeks
Result:	Significantly delayed the tumour growth.

体内研究

AG1024 (Tyrphostin AG 1024; 30 μg; i.p.; per day; for 2 weeks) significantly delays the tumour growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female nude mice (6-8 weeks old)
Dosage:	30 μg
Administration:	IP; per day; for 2 weeks
Result:	Significantly delayed the tumour growth.

体内研究

AG1024 (Tyrphostin AG 1024; 30 μg; i.p.; per day; for 2 weeks) significantly delays the tumour growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female nude mice (6-8 weeks old)
Dosage:	30 μg
Administration:	IP; per day; for 2 weeks
Result:	Significantly delayed the tumour growth.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 50 mg/mL (163.84 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2769 mL	16.3843 mL	32.7686 mL
5 mM	0.6554 mL	3.2769 mL	6.5537 mL
10 mM	0.3277 mL	1.6384 mL	3.2769 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (8.19 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (8.19 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液