Acrylodan|cas:86636-92-2|西域试剂

Acrylodan,95.01%

产品编号：Bellancom-W040230| CAS NO：86636-92-2| 分子式：C₁₅H₁₅NO| 分子量：225.29

Acrylodan 可与硫醇反应，对 Cys34 周围的结合口袋内的局部环境双极性和动力学敏感。

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-W040230		￥800.00	杭州北京（现货）

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Acrylodan

产品介绍

Acrylodan 可与硫醇反应，对 Cys³⁴ 周围的结合口袋内的局部环境双极性和动力学敏感。

生物活性

Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys³⁴.

体外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M⁻¹ cm⁻¹ for actin at 290 nm, 18,500 M⁻¹ cm⁻¹ or acrylodan at 385 nm, and 71,000 M⁻¹ cm⁻¹ for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (^AcrylAct1_NAT) is dramatically different than that of EDTA-unfolded ^AcrylAct1 (^AcrylAct1I3), which has a very similar emission spectrum to CCT-bound ^AcrylAct1.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 50 mg/mL (221.94 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.4387 mL	22.1936 mL	44.3872 mL
5 mM	0.8877 mL	4.4387 mL	8.8774 mL
10 mM	0.4439 mL	2.2194 mL	4.4387 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式